Iopamidol

Catalog No.S4532 Batch:S453201

Technical Data

Formula	C₁₇H₂₂I₃N₃O₈
Molecular Weight	777.09	CAS No.	60166-93-0
Solubility (25°C)*	In vitro	DMSO	100 mg/mL (128.68 mM)
		Water	100 mg/mL (128.68 mM)
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Iopamidol (Iopamiro, Isovue, Iopamiron, Niopam, Solutrast,B-15000,SQ-13396) is a non-ionic, water-soluble radiographic contrast agent.
In vitro	Iopamidol significantly decreases the rate of atrial contraction to a greater extent than either formulation of iodixanol. Iopamidol decreases papillary muscle force development more than the sodium formulation of iodixanol^[3].
In vivo	Iopamidol increases systolic blood pressure (SBP), mean arterial pressure (MAP), and peak left ventricular pressure (LVP). Iopamidol increases LVP and LV end diastolic pressure to a greater extent than the cationic formulation of iodixanol. Thus iopamidol affects cardiovascular parameters more than iodixanol^[3].

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines Primary chondrocytes Concentrations 102 mg/mL, 204 mg/mL, or 408 mg/mL Incubation Time 16 h Method Chondrocytes are plated on 96 well plates at a density of 1 x 10⁶ cells/cm² . After overnight incubation in standard tissue culture conditions, contrast agents (including iopamidol) are introduced to wells in varying concentrations. Following 16 hour incubation with PBS and contrast agents, chondrocyte viability is assessed using the Live/Dead stain kit and quantified using the CellTiter-Glo Luminescent Cell Viability Assay.

Cell Assay:^{^[2]}

Cell lines
Primary chondrocytes
Concentrations
102 mg/mL, 204 mg/mL, or 408 mg/mL
Incubation Time
16 h
Method
Chondrocytes are plated on 96 well plates at a density of 1 x 10⁶ cells/cm² . After overnight incubation in standard tissue culture conditions, contrast agents (including iopamidol) are introduced to wells in varying concentrations. Following 16 hour incubation with PBS and contrast agents, chondrocyte viability is assessed using the Live/Dead stain kit and quantified using the CellTiter-Glo Luminescent Cell Viability Assay.

References

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.