Datasheet

Biological Description

Specificity	Intestinal Alkaline Phosphatase Rabbit mAb detects endogenous levels of total Intestinal Alkaline Phosphatase protein.
Background	Intestinal alkaline phosphatase (IAP) is a membrane-bound, zinc-dependent metalloenzyme belonging to the alkaline phosphatase (AP) family, encoded in humans by the ALPI gene on chromosome 2. Structurally, it shares high amino acid sequence homology with placental AP, and also with tissue-nonspecific AP (TNAP), reflecting an evolutionary divergence between tissue-specific and nonspecific isoforms. IAP is predominantly expressed and secreted by enterocytes of the small intestine, with highest mRNA expression in the duodenum and maximal enzymatic activity in the terminal ileum; it is also released into the intestinal lumen and, to a lesser extent, the circulation, where it remains catalytically active. Functionally, IAP plays a central role in maintaining gut homeostasis by dephosphorylating and detoxifying pathogen-associated molecular patterns such as lipopolysaccharide (LPS), flagellin, unmethylated CpG DNA, and extracellular nucleotides (ATP, ADP, UDP), thereby dampening pro-inflammatory signaling. It supports commensal bacterial colonization, limits bacterial translocation across the epithelium, modulates duodenal pH and bicarbonate secretion, facilitates long-chain fatty acid absorption, and shapes microbiome composition in response to diet. Reduced IAP expression—seen in conditions like prematurity, starvation, inflammation, and certain surgical states—has been linked to dysbiosis, intestinal inflammation, and systemic sepsis risk, making recombinant or exogenous IAP a promising therapeutic candidate for inflammatory, infectious, and postoperative complications.

Specificity

Intestinal Alkaline Phosphatase Rabbit mAb detects endogenous levels of total Intestinal Alkaline Phosphatase protein.

Background

Intestinal alkaline phosphatase (IAP) is a membrane-bound, zinc-dependent metalloenzyme belonging to the alkaline phosphatase (AP) family, encoded in humans by the ALPI gene on chromosome 2. Structurally, it shares high amino acid sequence homology with placental AP, and also with tissue-nonspecific AP (TNAP), reflecting an evolutionary divergence between tissue-specific and nonspecific isoforms. IAP is predominantly expressed and secreted by enterocytes of the small intestine, with highest mRNA expression in the duodenum and maximal enzymatic activity in the terminal ileum; it is also released into the intestinal lumen and, to a lesser extent, the circulation, where it remains catalytically active. Functionally, IAP plays a central role in maintaining gut homeostasis by dephosphorylating and detoxifying pathogen-associated molecular patterns such as lipopolysaccharide (LPS), flagellin, unmethylated CpG DNA, and extracellular nucleotides (ATP, ADP, UDP), thereby dampening pro-inflammatory signaling. It supports commensal bacterial colonization, limits bacterial translocation across the epithelium, modulates duodenal pH and bicarbonate secretion, facilitates long-chain fatty acid absorption, and shapes microbiome composition in response to diet. Reduced IAP expression—seen in conditions like prematurity, starvation, inflammation, and certain surgical states—has been linked to dysbiosis, intestinal inflammation, and systemic sepsis risk, making recombinant or exogenous IAP a promising therapeutic candidate for inflammatory, infectious, and postoperative complications.

Usage Information

Application

WB, IHC

Dilution

WB	IHC
1:1000 - 1:10000	1:100 - 1:500

Reactivity

Mouse, Human

Source

Rabbit

MW

57 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/27083970/
https://pubmed.ncbi.nlm.nih.gov/25400448/

Intestinal Alkaline Phosphatase Rabbit mAb

Biological Description

Usage Information

References

Application Data