Insulin (human)

Catalog No.S6955 Batch:S695501

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Technical Data

Formula

C257H383N65O77S6
Molecular Weight 5807.57 CAS No. 11061-68-0
Solubility (25°C)* In vitro 0.01M HCl 13 mg/mL (2.23 mM)
Ethanol Insoluble
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Insulin (human) (Insulin regular) is a polypeptide hormone that regulates the level of glucose. Insulin is commonly used to treat hyperglycemia in patients with diabetes. Potency: 29 units/mg.
In vitro

Insulin, an inducer of AKT phosphorylation, reduces the levels of LC3-II, VP1 by the AKT-AMPKMAPK-MTOR pathway in SVV-infected cells.[2]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    BHK-21 cells and PK-15 cells

  • Concentrations

    200 ng/ml

  • Incubation Time

    1 h

  • Method

    BHK-21 cells and PK-15 cells are grown to 80% confluence in well plates and pretreated with insulin (200 ng/ml) for 1 h. The pretreated cells are then infected with SVV for 1 h. The cells are then washed with phosphate-buffered saline (PBS) and incubated with new medium containing the same chemical reagents. Cell viability in the presence of chemical reagents is tested.

Selleck's Insulin (human) has been cited by 6 publications

Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity [ Discov Oncol, 2023, 14(1):139] PubMed: 37498338
Artesunate alleviates 5-fluorouracil-induced intestinal damage by suppressing cellular senescence and enhances its antitumor activity [ Discov Oncol, 2023, 14(1):139] PubMed: 37498338
EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli [ Cell Death Dis, 2022, 13(4):351] PubMed: 35428350
Somatostatin receptor ligands suppressed proliferation and lipogenesis in 3T3-L1 preadipocytes [ Basic Clin Pharmacol Toxicol, 2022, 131(3):174-188] PubMed: 35688794
PI3K/AKT1 Signaling Pathway Mediates Sinomenine-Induced Hepatocellular Carcinoma Cells Apoptosis: An in Vitro and in Vivo Study [ Biol Pharm Bull, 2022, 45(5):614-624] PubMed: 35491166
Synergetic contributions of viral VP1, VP3, and 3C to activation of the AKT-AMPK-MAPK-MTOR signaling pathway for Seneca Valley virus-induced autophagy [ J Virol, 2021, JVI0155021] PubMed: 34757844

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.