Imidazole Ketone Erastin (IKE)

Catalog No.S8877 Batch:S887704

Technical Data

Formula

C₃₅H₃₅ClN₆O₅

Molecular Weight

655.14

CAS No.

1801530-11-9

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (152.63 mM)

Ethanol

100 mg/mL (152.63 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

IKE(Imidazole ketone erastin) is a potent, selective, and metabolically stable system xc– inhibitor and inducer of ferroptosis.

Targets

Ferroptosis ^[1]

system xc– ^[1]

In vitro

Imidazole ketone erastin (IKE) is an erastin analog with nanomolar potency, high metabolic stability, and intermediate water solubility. Its treatment potently reduces DLBCL cell number.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines DLBCL cell line Concentrations 0-100 μM Incubation Time 24 h Method DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed.
Animal Study:^{^[1]}	Animal Models Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice Dosages 50 mg/kg Administration IP, IV and oral administration

Cell Assay:^{^[1]}

Cell lines
DLBCL cell line
Concentrations
0-100 μM
Incubation Time
24 h
Method
DLBCL cells are plated at 10,000 cells per well in white 384-well plates (32 μL per well) in technical duplicates and incubated overnight. The cells are then treated with 8 μL medium containing a two-fold dilution series of vehicle (DMSO), IKE (starting from 100 μM) with or without Fer-1 (starting from 200 μM). After 24 h incubation, 40 μL of 50% CellTiter-Glo / 50% cell culture medium is added to each well and incubated at room temperature with shaking for 15 min. Luminescence is measured. All cell viability data are normalized to the DMSO vehicle condition. Experiments are performed three independent times with different passages for each cell line. From these data, dose-response curves and IC50 values are computed.

Animal Study:^{^[1]}

Animal Models
Non-obese diabetic/ severe combined immunodeficiency (NOD/SCID) mice
Dosages
50 mg/kg
Administration
IP, IV and oral administration

References

https://pubmed.ncbi.nlm.nih.gov/30799221/

Selleck's Imidazole Ketone Erastin (IKE) Has Been Cited by 49 Publications

mTORC1 activity suppresses ferroptosis through a SCARB1-dependent HDL-tocopherol uptake pathway [ Mol Cell, 2026, 86(8):1546-1559.e8]	PubMed: 41997112
The HIF-2 transcription factor mediates resistance to ferroptosis in pancreatic cancer [ Mol Cell, 2026, 86(7):1260-1274.e4]	PubMed: 41932308
FAK/SRC-JNK axis promotes ferroptosis via upregulating ACSL4 expression [ Cell Death Dis, 2026, 17(1)328]	PubMed: 41862445
Dual inhibition of xCT and GGCT induces ferroptosis in glioblastoma cells by depleting cysteine and disrupting redox homeostasis [ Cell Death Discov, 2026, 12(1):249]	PubMed: 41986303
Lactate-driven pyrimidine synthesis promotes ferroptosis resistance in hepatocellular carcinoma [ Mol Biomed, 2026, 7(1)53]	PubMed: 41996004
SLC7A11 is an unconventional H+ transporter in lysosomes [ Cell, 2025, S0092-8674(25)00406-4]	PubMed: 40280132
Endoplasmic reticulum-mitochondria contacts are prime hotspots of phospholipid peroxidation driving ferroptosis [ Nat Cell Biol, 2025, 27(6):902-917]	PubMed: 40514428
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states [ Nat Metab, 2025, 7(9):1851-1870]	PubMed: 40957995
USP10 Inhibits Ferroptosis via Deubiquinating POLR2A in Head and Neck Squamous Cell Carcinoma [ Adv Sci (Weinh), 2025, 12(36):e12271]	PubMed: 40605431
Targeting pancreatic cancer glutamine dependency confers vulnerability to GPX4-dependent ferroptosis [ Cell Rep Med, 2025, 6(2):101928]	PubMed: 39879992

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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