IL-1RA Rabbit mAb

Catalog No.: F2174

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Biological Description

Specificity IL-1RA Rabbit mAb recognizes endogenous levels of total IL-1RA protein.
Background IL-1RA (Interleukin-1 receptor antagonist) is a naturally occurring antagonist cytokine within the IL-1 family that binds competitively to the interleukin-1 type I receptor (IL-1RI) without triggering downstream signaling. IL-1Ra shares the conserved β-trefoil fold characteristic of IL-1 family cytokines, consisting of 12 β-strands and two short 3₁₀ helices, similar to IL-1α and IL-1β. However, key conformational differences in the β4/5 and β11/12 loops prevent IL-1Ra from recruiting the secondary receptor IL-1 receptor accessory protein (IL-1RAcP), thereby blocking the formation of the active ternary signaling complex essential for pro-inflammatory signaling. By occupying IL-1RI, IL-1Ra competitively inhibits binding of the agonist cytokines IL-1α and IL-1β, preventing receptor conformational changes and downstream activation of inflammatory pathways such as NF-κB. This antagonistic mechanism is critical for regulating immune responses, maintaining immune homeostasis, and preventing excessive inflammation and autoinflammatory diseases.

Usage Information

Application WB Dilution
WB
1:10000 - 1:50000
Reactivity Human
Source Rabbit MW 20 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data