IKB epsilon Rabbit Recombinant mAb Datasheet

Description

Specificity	IKB epsilon Rabbit Recombinant mAb detects endogenous level of total IKB epsilon.
Background	Members of the nuclear factor of kappa B (NF-κB) transcription factor family, p50, p52, p65 (RelA), c-Rel and RelB, are maintained as inactive homo- or heterodimers in the cytoplasm by inhibitors of kappa B (IκBs). IκB degradation permits nuclear translocation of NF-κB and induction of genes responsible for many cellular responses including inflammation, survival and differentiation. IκBε differs from IκBs -α and -β structurally. IκBα tends to bind p65/p50, while IκBs-ε and -β prefer c-Rel and p65. IκBε is expressed in T and B lymphocytes. It regulates genes transactivated by heterodimers of c-Rel and homodimers of RelA. Functionally, IκBε and IκBα both contribute to post-induction repression of transcription by removing NF-κB from the nucleus.

Specificity

IKB epsilon Rabbit Recombinant mAb detects endogenous level of total IKB epsilon.

Background

Members of the nuclear factor of kappa B (NF-κB) transcription factor family, p50, p52, p65 (RelA), c-Rel and RelB, are maintained as inactive homo- or heterodimers in the cytoplasm by inhibitors of kappa B (IκBs). IκB degradation permits nuclear translocation of NF-κB and induction of genes responsible for many cellular responses including inflammation, survival and differentiation. IκBε differs from IκBs -α and -β structurally. IκBα tends to bind p65/p50, while IκBs-ε and -β prefer c-Rel and p65. IκBε is expressed in T and B lymphocytes. It regulates genes transactivated by heterodimers of c-Rel and homodimers of RelA. Functionally, IκBε and IκBα both contribute to post-induction repression of transcription by removing NF-κB from the nucleus.

Usage Information

Application

WB, IHC,ELISA

Dilution

WB	IHC
1:1000	1:50

Reactivity

Human

MW (kDa)

45kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

[1] Clark JM, et al. PLoS One. 2011, 6(9):e24504.

Application Data

WB

Validated by Selleck

IHC