IBMX

Catalog No.S5836 Batch:S583601

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Technical Data

Formula

C10H14N4O2
Molecular Weight 222.24 CAS No. 28822-58-4
Solubility (25°C)* In vitro DMSO 44 mg/mL (197.98 mM)
Ethanol 7 mg/mL (31.49 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description IBMX (Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine) is a nonspecific inhibitor of phosphodiesterase (PDE) with IC50 values of 6.5±1.2, 26.3±3.9 and 31.7±5.3 μM for PDE3, 4 and 5 respectively. It may enhance the intracellular cAMP levels and also acts as an adenosine (A1) receptor antagonist.
Targets
PDE3 [1] PDE4 [1] PDE5 [1]
6.5 μM 26.3 μM 31.7 μM
In vitro

IBMX rescues PGE2-induced phosphorylation of AKT inhibited in cells from the EP4LysM mice, which protects against cAMP degradation.[3]
In vivo

IBMX decreases the liver glycogen storage in both species of mice and hyperglycemic rat.[4]

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    Bone marrow macrophages of Ep4fl/fl and Ep4LysM mice

  • Concentrations

    1 mM

  • Incubation Time

    3 h

  • Method

    The cells are treated either with osteoclastogenic media (10 ng/mL M-CSF and 50 ng/mL RANKL) alone, with PGE2 (100 nM), or PGE2 with IBMX (1 mM) for 3 h. A BCA Protein Assay Kit is used to determine the protein concentration.
Animal Study:

[4]
  • Animal Models

    Male mice (25-35 g), adult Wistar rats (250-350 g) of hyperglycemic model

  • Dosages

    1 mg/kg dosage twice a day

  • Administration

    s.c.

Selleck's IBMX has been cited by 13 publications

LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexia [ J Hematol Oncol, 2023, 16(1):30] PubMed: 36973755
Kinase-Modulated Bioluminescent Indicators Enable Noninvasive Imaging of Drug Activity in the Brain [ ACS Cent Sci, 2023, 9(4):719-732] PubMed: 37122464
Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion [ Phytomedicine, 2023, 118:154942] PubMed: 37421767
PTHrP Modulates the Proliferation and Osteogenic Differentiation of Craniofacial Fibrous Dysplasia-Derived BMSCs [ Int J Mol Sci, 2023, 24(8)7616] PubMed: 37108778
Ursodeoxycholic acid reduces antitumor immunosuppression by inducing CHIP-mediated TGF-β degradation [ Nat Commun, 2022, 13(1):3419] PubMed: 35701426
PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis [ Bone Res, 2022, 10(1):27] PubMed: 35260562
Rapid recovery of male cats with postrenal acute kidney injury by treating with allogeneic adipose mesenchymal stem cell-derived extracellular vesicles [ Stem Cell Res Ther, 2022, 13(1):379] PubMed: 35902973
Rapamycin improves the quality and developmental competence of in vitro matured oocytes in aged mice and humans [ Aging (Albany NY), 2022, 14(22):9200-9209] PubMed: 36441531
Rapamycin improves the quality and developmental competence of mice oocytes by promoting DNA damage repair during in vitro maturation [ Reprod Biol Endocrinol, 2022, 20(1):67] PubMed: 35436937
Pharmacological and molecular dynamics analyses of differences in inhibitor binding to human and nematode PDE4: Implications for management of parasitic nematodes [ PLoS One, 2019, 14(3):e0214554] PubMed: 30917179

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.