GsMTx4

Catalog No.P1205 Batch:P120502

Technical Data

Formula	C₁₈₅H₂₇₃N₄₉O₄₅S₆
Molecular Weight	4095.86	CAS No.	1209500-46-8
Solubility (25°C)*	In vitro	Water	82 mg/mL (20.02 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

GsMTx4 (Grammostola spatulata mechanotoxin 4, M-theraphotoxin-Gr1a, M-TRTX-Gr1a) is a spider venom peptide that inhibits cationic mechanosensitive channels (MSCs).

Targets

MSCs ^[1]

In vitro

Pretreatment of GsMTx4 and BAPTA-AM suppress the Yoda1-induced accumulation of HIF-1alpha, NFATC1, and NFATC3 proteins and also suppress Yoda1-induced VEGF-A expression at the mRNA and protein levels.^[2]

Density

95.0~105.0%

Protocol (from reference)

Cell Assay:^{^[3]}	Cell lines BMDMs, RAW264.7 cells Concentrations 5, 10 µM Incubation Time 30 min Method To study the effect of mechanical stretching, BMDMs or RAW264.7 cells were treated with Yoda1, with or without pretreatment with inhibitors for 30 min. The inhibitors included cyclosporin A, FK506, echinomycin, GsMTx4, BAPTA-AM and RN-1734. In some experiments, BMDMs were treated with GSK1016790A. (Data sourced from selleck products)
Animal Study:^{^[3]}	Animal Models Male C57BL/6 mice Dosages 0.8 mg/kg Administration i.p.

Cell Assay:^{^[3]}

Cell lines
BMDMs, RAW264.7 cells
Concentrations
5, 10 µM
Incubation Time
30 min
Method
To study the effect of mechanical stretching, BMDMs or RAW264.7 cells were treated with Yoda1, with or without pretreatment with inhibitors for 30 min. The inhibitors included cyclosporin A, FK506, echinomycin, GsMTx4, BAPTA-AM and RN-1734. In some experiments, BMDMs were treated with GSK1016790A.

(Data sourced from selleck products)

Animal Study:^{^[3]}

Animal Models
Male C57BL/6 mice
Dosages
0.8 mg/kg
Administration
i.p.

References

[4] Xu H, et al. FASEB J. 2022 Aug;36(8):e22423.

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Selleck's GsMTx4 has been cited by 4 publications

Enriched environment ameliorates fear memory impairments induced by sleep deprivation via inhibiting PIEZO1/calpain/autophagy signaling pathway in the basal forebrain [ CNS Neurosci Ther, 2023, 10.1111/cns.14365]	PubMed: 37485782
An EPHB4-RASA1 signaling complex inhibits shear stress-induced Ras-MAPK activation in lymphatic endothelial cells to promote the development of lymphatic vessel valves [ bioRxiv, 2023, 2023.11.22.568378]	PubMed: 38045382
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4): 1621-1638]	PubMed: N/A
Piezo1-mediated mechanosensation in bone marrow macrophages promotes vascular niche regeneration after irradiation injury [ Theranostics, 2022, 12(4):1621-1638]	PubMed: 35198061

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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