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Specificity | GRK2 Rabbit Recombinant mAb detects endogenous level of total GRK2. |
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Background | GRK2 is a ubiquitous member of the G protein-coupled receptor kinase (GRK) family that appears to play a central, integrative role in signal transduction cascades. GRKs participate together with arrestins in the regulation of G protein-coupled receptors (GPCR), a family of hundreds of membrane proteins of key physiological and pharmacological importance, by triggering receptor desensitization from G proteins and GPCR internalization, and also by helping assemble macromolecular signalosomes in the receptor environment acting as agonist-regulated adaptor scaffolds, thus contributing to signal propagation. GRK family members can be subdivided into three main groups based on sequence homology: visual GRK subfamily (GRK1 and GRK7), the β-adrenergic receptor kinase subfamily (GRK2/GRK3) and the GRK4 subfamily (GRK4, GRK5 and GRK6). GRK2, 3, 5 and 6 are ubiquitously expressed in mammalian tissues. GRK2 can phosphoryate non-GPCR substrates and display a complex network of functional interactions. GRK2 contributes to orchestrate G2/M checkpoint mechanisms, helping to restrict the apoptotic fate of arrested cells. |
Application | WB, IHC, IF,ELISA | ||||||
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Dilution |
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Reactivity | Human Mouse Rat | ||||||
MW (kDa) | 80kDa | ||||||
Source | Rabbit | ||||||
Concentration | 1mg/ml | ||||||
Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||||||
Storage (From the date of receipt) |
Store at –20°C. |
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WB
IHC
IF