Datasheet

Biological Description

Specificity	GOPC Antibody [F21D16] detects endogenous levels of total GOPC protein.
Background	GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein), also known as FIG or PIST, is a pivotal scaffolding protein localized primarily to the trans-Golgi network, characterized by two N-terminal coiled-coil domains and a C-terminal PDZ domain. Its main functional role centers on mediating vesicular trafficking and regulating the sorting and surface expression of various membrane proteins, including ion channels, receptors, and adhesion molecules. GOPC interacts with golgin-160 and the Q-SNARE protein syntaxin 6 through its coiled-coil domains, facilitating cargo transport in secretory and endocytic pathways. The PDZ domain specifically binds to the C-termini of target proteins such as Frizzled receptors, neuroligins, and CFTR, thus influencing processes like cell signaling, tight junction integrity, and receptor recycling. GOPC is essential in maintaining epithelial cell polarity, regulating tight junctions, and modulating cellular homeostasis. Moreover, GOPC gains pathological significance in glioblastoma, where fusion with ROS tyrosine kinase leads to constitutive kinase activity driving oncogenesis.

Specificity

GOPC Antibody [F21D16] detects endogenous levels of total GOPC protein.

Background

GOPC (Golgi-associated PDZ and coiled-coil motif-containing protein), also known as FIG or PIST, is a pivotal scaffolding protein localized primarily to the trans-Golgi network, characterized by two N-terminal coiled-coil domains and a C-terminal PDZ domain. Its main functional role centers on mediating vesicular trafficking and regulating the sorting and surface expression of various membrane proteins, including ion channels, receptors, and adhesion molecules. GOPC interacts with golgin-160 and the Q-SNARE protein syntaxin 6 through its coiled-coil domains, facilitating cargo transport in secretory and endocytic pathways. The PDZ domain specifically binds to the C-termini of target proteins such as Frizzled receptors, neuroligins, and CFTR, thus influencing processes like cell signaling, tight junction integrity, and receptor recycling. GOPC is essential in maintaining epithelial cell polarity, regulating tight junctions, and modulating cellular homeostasis. Moreover, GOPC gains pathological significance in glioblastoma, where fusion with ROS tyrosine kinase leads to constitutive kinase activity driving oncogenesis.

Usage Information

Application

WB

Dilution

WB

1:1000

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

59 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/25616555/
https://pubmed.ncbi.nlm.nih.gov/16882988/

GOPC Antibody [F21D16]

Biological Description

Usage Information

References

Application Data