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Description
| Specificity | GCLC Rabbit Recombinant mAb detects endogenous level of total GCLC. |
|---|---|
| Background | Glutamate cysteine ligase (GCL) is a holoenzyme comprising a ∼73 kDa catalytic subunit (GCLC), 637 amino acids in length and a ∼31 kDa modifier (GCLM) subunit, 274 amino acids in length. The specific activity of GCL is a major determinant of the rate of GSH (glutathione) synthesis and homeostasis. GCLC (Glutamate-cysteine ligase catalytic subunit) is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The GCLC active site facilitates the formation of the peptide bond between cysteine's amino group and glutamate's γ-carboxyl group. |
Usage Information
| Application | WB,ELISA | ||
|---|---|---|---|
| Dilution |
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| Reactivity | Human Rat | ||
| MW (kDa) | 73kDa | ||
| Source | Rabbit | ||
| Concentration | 1mg/ml | ||
| Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| Storage (From the date of receipt) |
Store at –20°C. |
Application Data
WB