Fer-1 (Ferrostatin-1)

Catalog No.S7243 Batch:S724301

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Technical Data

Formula

C15H22N2O2
Molecular Weight 262.35 CAS No. 347174-05-4
Solubility (25°C)* In vitro DMSO 52 mg/mL (198.2 mM)
Ethanol 52 mg/mL (198.2 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Fer-1 (Ferrostatin-1) is a potent and selective inhibitor of ferroptosis with EC50 of 60 nM.
Targets
Ferroptosis [1]
(HT-1080 cells)
60 nM(EC50)
In vitro

Ferrostatin-1 (2 μM) prevents erastin-induced ferroptosis in cancer cells, as well as glutamate-induced cell death in postnatal rat brain slices. Ferrostatin-1 is a lipid ROS scavenger, with the N-cyclohexyl moiety serving as a lipo-philic anchor within biological membranes. Ferrostatin-1 does not inhibit extracellular signal -regulated kinase (ERK) phos-phorylation or arrest the proliferation of HT-1080 cells, suggesting that it does not inhibit the MEK/ERK pathway, chelate iron, or inhibit protein synthesis. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 readily oxidizes the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) under cell-free conditions. [1]
In vivo

Ferrostatin-1 (Fer-1), a synthetic antioxidant, is a potent and selective inhibitor of ferroptosis. It acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death.[2]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HT-1080 cells

  • Concentrations

    60 nM (EC50)

  • Incubation Time

    24 h

  • Method

    Erastin treated cells were incubated with ferrostatin-1 at 37˚C for 24 hr and viability was assessed using Alamar Blue assay.
Animal Study:

[2]
  • Animal Models

    C57BL/6 mice

  • Dosages

    10 mg/kg

  • Administration

    i.n.

Customer Product Validation

Data from [Data independently produced by , , Hepatology, 2016, 64(2):488-500]

Data from [Data independently produced by , , Cancer Res, 2017, 77(8):2064-2077]

Data from [Data independently produced by , , Oncogene, 2015, 10.1038/onc.2015.32]

Data from [Data independently produced by , , Mol Carcinog, 2018, 57(11):1566-1576]

Selleck's Fer-1 (Ferrostatin-1) has been cited by 438 publications

7-Dehydrocholesterol dictates ferroptosis sensitivity [ Nature, 2024, 626(7998):411-418.] PubMed: 38297130
Targeting of vulnerabilities of drug-tolerant persisters identified through functional genetics delays tumor relapse [ Cell Rep Med, 2024, 5(3):101471] PubMed: 38508142
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R [ Redox Biol, 2024, 72:103154] PubMed: 38626575
Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis [ Redox Biol, 2024, 69:103002] PubMed: 38142583
FTO ameliorates doxorubicin-induced cardiotoxicity by inhibiting ferroptosis via P53-P21/Nrf2 activation in a HuR-dependent m6A manner [ Redox Biol, 2024, 70:103067] PubMed: 38316068
Meningioma achieves malignancy and erastin-induced ferroptosis resistance through FOXM1-AURKA-NRF2 axis [ Redox Biol, 2024, 72:103137] PubMed: 38642502
Protein phosphatase 1 regulatory subunit 15 A promotes translation initiation and induces G2M phase arrest during cuproptosis in cancers [ Cell Death Dis, 2024, 15(2):149] PubMed: 38365764
Salidroside pretreatment alleviates ferroptosis induced by myocardial ischemia/reperfusion through mitochondrial superoxide-dependent AMPKα2 activation [ Phytomedicine, 2024, 155365] PubMed: none
Vitamin B6 alleviates chronic sleep deprivation-induced hippocampal ferroptosis through CBS/GSH/GPX4 pathway [ Biomed Pharmacother, 2024, 174:116547] PubMed: 38599059
Blocking CIRP protects against acute pancreatitis by improving mitochondrial function and suppressing pyroptosis in acinar cells [ Cell Death Discov, 2024, 10(1):156] PubMed: 38538578

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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