VX-445 (Elexacaftor)

Catalog No.S8851 Batch:S885101

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Technical Data

Formula

C26H34F3N7O4S
Molecular Weight 597.65 CAS No. 2216712-66-0
Solubility (25°C)* In vitro DMSO 100 mg/mL (167.32 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 2.500mg/ml (4.18mM) Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween-80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O Dilute to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5%DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 5.000mg/ml (8.37mM) Taking the 1 mL working solution as an example, add 50 μL of 100 mg/mL clarified DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Elexacaftor (VX-445) is a next-generation cystic fibrosis transmembrane conductance regulator (CFTR) corrector.This is a compound which is not chiral purity.
Targets
CFTR [1]
In vitro

The pendrin-dependent increase in cystic fibrosis transmembrane conductance regulator (CFTR) current is additive with the CFTR modulator elexacaftor.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Human bronchial epithelial cells from DeltaF508/DeltaF508 Cystic Fibrosis patients (CF HBECs)

  • Concentrations

    3 µM

  • Incubation Time

    48 h

  • Method

    Using Chamber recordings from DeltaF508/DeltaF508 CF HBEC epithelia were treated with the LIE triple combination for 48 h plus and minus 10 ng/mL IL-13 and/or 10 micromol/L niflumic acid (for 30 minutes) again. Transepithelial current (Isc) and conductance (Gt) were measured.

    (Data sourced from selleck products)

References

  • https://pubmed.ncbi.nlm.nih.gov/30334692/
  • https://pubmed.ncbi.nlm.nih.gov/32923987/

Selleck's VX-445 (Elexacaftor) Has Been Cited by 90 Publications

Uncovering Cystic Fibrosis Carrier: Insights From a Heterozygous CFTR-F508del Rabbit Model [ Int Forum Allergy Rhinol, 2026, 10.1002/alr.70128] PubMed: 41758072
Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations [ Mol Ther Oncol, 2026, 34(2):201173] PubMed: 41939791
Altered functional interactions between CFTR disease mutants ΔF508 and G551D and the protein kinase A catalytic subunit [ J Physiol, 2026, 604(8):3344-3362] PubMed: 41920076
Investigation of HE4 expression concerning epithelial-mesenchymal transition (EMT) in cystic fibrosis epithelial cells [ J Cyst Fibros, 2026, S1569-1993(26)00086-X] PubMed: 41936498
CFTR rescue in W1282X cystic fibrosis patient-derived intestinal organoids (PDIOs) mediated by translational readthrough-inducing drugs (TRIDs) [ Genet Med Open, 2026, 4:103472] PubMed: 41492359
ACE-tRNAs are a platform technology for suppressing nonsense mutations that cause cystic fibrosis [ Nucleic Acids Res, 2025, 53(13)gkaf675] PubMed: 40650978
Antisense oligonucleotide targeting the E3 ligase RFFL potentiates CFTR modulator efficacy in CF primary bronchial epithelial cells [ Mol Ther Nucleic Acids, 2025, 36(4):102756] PubMed: 41323797
Transcriptomic and functional responses of the cystic fibrosis airway epithelium to CFTR modulator therapy [ JCI Insight, 2025, 10(21)e196018] PubMed: 41212059
Endometrium-derived organoids from cystic fibrosis patients and mice as new models to study disease-associated endometrial pathobiology [ Cell Mol Life Sci, 2025, 82(1):109] PubMed: 40074868
CFTR negatively reprograms Th2 cell responses, and CFTR potentiation restrains allergic airway inflammation [ JCI Insight, 2025, 10(9)e191098] PubMed: 40131363

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.