Elamipretide (MTP-131, SS-31)

Catalog No.S9803 Batch:S980303

Technical Data

Formula	C₃₂H₄₉N₉O₅
Molecular Weight	639.79	CAS No.	736992-21-5
Solubility (25°C)*	In vitro	Water	100 mg/mL (156.3 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Elamipretide (MTP-131, SS-31) is a cytochrome c peroxidase inhibitor. Elamipretide improves mitochondrial dysfunction, synaptic and memory impairment induced by lipopolysaccharide in mice.

Targets

cytochrome c peroxidase ^[1]

In vitro

SS-31 binds with high affinity to cardiolipin, an anionic phospholipid expressed on the inner mitochondrial membrane that is required for cristae formation. In addition, the SS-31/cardiolipin complex inhibits cytochrome c peroxidase activity, which catalyzes cardiolipin peroxidation and results in mitochondrial damage during ischemia, by protecting its heme iron.^[1]

In vivo

Pretreatment of rats with SS-31 protects cristae membranes during renal ischemia and prevents mitochondrial swelling. Prompt recovery of ATP on reperfusion leads to rapid repair of ATP-dependent processes, such as restoration of the actin cytoskeleton and cell polarity. Rapid recovery of ATP also inhibits apoptosis, protects tubular barrier function, and mitigates renal dysfunction.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines feline kidney cells Concentrations 0.1-10 μM Incubation Time 1 hour Method Intracellular targeting of SS-31 is determined by incubating feline kidney cells, grown in biotin-free media for 24 hours, with biotinylated SS-31 for 1 hour. Cells are then fixed with 4% paraformaldehyde, permeabilized with 0.1% triton, and treated with Streptavidin-AlexaFluor594 for 30 minutes at room temperature. Fluorescence staining is observed by wide-field fluorescent microscope.
Animal Study:^{^[1]}	Animal Models male Sprague–Dawley rats Dosages 2.0 mg/kg Administration SC

Cell Assay:^{^[1]}

Cell lines
feline kidney cells
Concentrations
0.1-10 μM
Incubation Time
1 hour
Method
Intracellular targeting of SS-31 is determined by incubating feline kidney cells, grown in biotin-free media for 24 hours, with biotinylated SS-31 for 1 hour. Cells are then fixed with 4% paraformaldehyde, permeabilized with 0.1% triton, and treated with Streptavidin-AlexaFluor594 for 30 minutes at room temperature. Fluorescence staining is observed by wide-field fluorescent microscope.

Animal Study:^{^[1]}

Animal Models
male Sprague–Dawley rats
Dosages
2.0 mg/kg
Administration
SC

References

Selleck's Elamipretide (MTP-131, SS-31) has been cited by 6 publications

Enolase of Streptococcus suis serotype 2 promotes biomolecular condensation of ribosomal protein SA for HBMECs apoptosis [ BMC Biol, 2024, 22(1):33]	PubMed: 38331785
NINJ1 regulates ferroptosis via xCT antiporter interaction and CoA modulation [ bioRxiv, 2024, 2024.02.22.581432]	PubMed: 38464226
Targeting mitochondria in the aged cerebral vasculature with SS-31, a proteomic study of brain microvessels [ Geroscience, 2023, 10.1007/s11357-023-00845-y]	PubMed: 37458933
Mycophenolic Acid Induces the Intestinal Epithelial Barrier Damage through Mitochondrial ROS [ Oxid Med Cell Longev, 2022, 2022:4195699]	PubMed: 35847589
High-Fat Diet-Induced Mitochondrial Dysfunction Promotes Genioglossus Injury - A Potential Mechanism for Obstructive Sleep Apnea with Obesity [ Nat Sci Sleep, 2021, 13:2203-2219]	PubMed: 34992480
Skeletal muscle NOX4 is required for adaptive responses that prevent insulin resistance [ Sci Adv, 2021, 7(51):eabl4988]	PubMed: 34910515

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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