Datasheet

Biological Description

Specificity	DYKDDDDK Tag Rabbit mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody, fused to either the amino-terminus or carboxy-terminus of the target protein.
Background	The DYKDDDDK tag, commonly known as the FLAG tag, is an eight–amino acid epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), widely used in molecular biology for protein purification, detection, and expression studies. This tag is genetically fused to either the N-terminal or C-terminal of a target protein using recombinant DNA technology, and its small size minimizes interference with the protein's function. The FLAG tag allows highly specific purification via affinity chromatography by binding to monoclonal antibodies, such as anti-FLAG M2 (position-insensitive) or M1 (N-terminal specific and calcium-dependent). Proteins fused with FLAG can be eluted from the matrix using a low pH buffer or excess FLAG peptide, and the tag can be removed enzymatically at a protease cleavage site (e.g., enterokinase). Tandem FLAG tags (e.g., 3xFLAG or modified sequences like DYKDHDG) are used for enhanced binding and detection. The FLAG tag is extensively applied in assays such as western blotting, ELISA, immunoprecipitation, flow cytometry, immunofluorescence, and SDS-PAGE, particularly in mammalian expression systems, and can also be combined with other epitope tags (e.g., HA, His, Myc) for multi-functional studies.

Specificity

DYKDDDDK Tag Rabbit mAb detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody, fused to either the amino-terminus or carboxy-terminus of the target protein.

Background

The DYKDDDDK tag, commonly known as the FLAG tag, is an eight–amino acid epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), widely used in molecular biology for protein purification, detection, and expression studies. This tag is genetically fused to either the N-terminal or C-terminal of a target protein using recombinant DNA technology, and its small size minimizes interference with the protein's function. The FLAG tag allows highly specific purification via affinity chromatography by binding to monoclonal antibodies, such as anti-FLAG M2 (position-insensitive) or M1 (N-terminal specific and calcium-dependent). Proteins fused with FLAG can be eluted from the matrix using a low pH buffer or excess FLAG peptide, and the tag can be removed enzymatically at a protease cleavage site (e.g., enterokinase). Tandem FLAG tags (e.g., 3xFLAG or modified sequences like DYKDHDG) are used for enhanced binding and detection. The FLAG tag is extensively applied in assays such as western blotting, ELISA, immunoprecipitation, flow cytometry, immunofluorescence, and SDS-PAGE, particularly in mammalian expression systems, and can also be combined with other epitope tags (e.g., HA, His, Myc) for multi-functional studies.

Usage Information

Application

WB, IP, IHC, IF, F, ChIP

Dilution

WB	IP	IHC	IF	FCM	CHIP
1:1000	1:50	1:400 - 1:1600	1:400 - 1:1600	1:800 - 1:3200	1:50

Reactivity

All

Source

Rabbit

MW

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

[1] Brizzard BL, et al. Biotechniques. 1994 Apr;16(4):730-5.

DYKDDDDK Tag Rabbit mAb

Biological Description

Usage Information

References

Application Data