CXCR4 Antibody [G11H20]

Catalog No.: F2178

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Biological Description

Specificity CXCR4 Antibody [G11H20] detects endogenous levels of total CXCR4 protein.
Background CXCR4, or C-X-C chemokine receptor type 4, is a G protein-coupled receptor (GPCR) classified within the chemokine receptor family. It is broadly expressed in tissues including immune cells (T and B lymphocytes), the bone marrow, brain, and heart, where it mediates responses essential for cell migration and proliferation by binding to its specific ligand CXCL12 (stromal cell-derived factor 1). CXCR4 consists of seven transmembrane alpha-helices characteristic of GPCRs, with extracellular loops and an N-terminal domain crucial for high-affinity ligand binding. Conserved disulfide bonds stabilize these extracellular components to shape the chemokine binding pocket, which operates according to a "two-site" model involving the chemokine core domain and its N-terminal signaling trigger. CXCR4 regulates hematopoiesis, immune cell trafficking, angiogenesis, and tissue regeneration through activating signaling pathways, including PI3K/AKT and MAPK. It also guides progenitor cell homing to the bone marrow and coordinates immune surveillance. CXCR4 is implicated in cancer metastasis via tumor cell migration and invasion, and serves as a critical HIV co-receptor facilitating viral entry into CD4+ T cells.

Usage Information

Application WB, IHC, IF, FCM Dilution
WB IHC IF FCM
1:100 1:500 1:550 1:1900
Reactivity Mouse, Rat, Human
Source Rabbit Monoclonal Antibody MW 39 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

References

  • https://pubmed.ncbi.nlm.nih.gov/32983169/
  • https://pubmed.ncbi.nlm.nih.gov/20929726/

Application Data