CtBP1 Antibody [J24B2] Datasheet

Biological Description

Specificity	CtBP1 Antibody [J24B2] detects endogenous levels of total CtBP1 protein.
Background	CtBP1 (C‑terminal‑binding protein 1) is a transcriptional corepressor and NAD‑dependent dehydrogenase that belongs to the CtBP family alongside its close homologue CtBP2 and is widely expressed in tissues involved in development, metabolism, and epithelial homeostasis. It contains conserved NAD‑binding and substrate‑binding domains that couple its enzymatic activity to the cellular redox state, with NADH levels influencing its conformation and interaction with partner proteins, and features dimerization and multimerization interfaces that support assembly of larger repressor complexes with other transcriptional regulators. CtBP1 binds a broad set of transcriptional repressors that contain PXDLS‑like motifs, including PRDM16, Snail family factors, and other zinc‑finger‑containing regulators, and functions within complexes that recruit histone deacetylases and other chromatin‑modifying enzymes to target gene promoters. CtBP1 interacts with PRDM16 and C/EBPα‑linked regulatory circuits that repress white‑fat‑associated genes and influence the brown‑fat differentiation program, positioning it as a redox‑sensitive component of adipogenic identity and energy‑balance signaling. CtBP1 regulates epithelial and tumor‑suppressor loci, including Brca1 and E‑cadherin, whose promoters are bound by CtBP1‑containing complexes so that elevated CtBP1 activity correlates with transcriptional downregulation of these genes and reduced epithelial integrity. In breast and other carcinomas, nuclear CtBP1 is frequently detected at elevated levels and is associated with reduced Brca1 and E‑cadherin expression, increased cell migration, and more aggressive disease phenotypes.

Specificity

CtBP1 Antibody [J24B2] detects endogenous levels of total CtBP1 protein.

Background

CtBP1 (C‑terminal‑binding protein 1) is a transcriptional corepressor and NAD‑dependent dehydrogenase that belongs to the CtBP family alongside its close homologue CtBP2 and is widely expressed in tissues involved in development, metabolism, and epithelial homeostasis. It contains conserved NAD‑binding and substrate‑binding domains that couple its enzymatic activity to the cellular redox state, with NADH levels influencing its conformation and interaction with partner proteins, and features dimerization and multimerization interfaces that support assembly of larger repressor complexes with other transcriptional regulators. CtBP1 binds a broad set of transcriptional repressors that contain PXDLS‑like motifs, including PRDM16, Snail family factors, and other zinc‑finger‑containing regulators, and functions within complexes that recruit histone deacetylases and other chromatin‑modifying enzymes to target gene promoters. CtBP1 interacts with PRDM16 and C/EBPα‑linked regulatory circuits that repress white‑fat‑associated genes and influence the brown‑fat differentiation program, positioning it as a redox‑sensitive component of adipogenic identity and energy‑balance signaling. CtBP1 regulates epithelial and tumor‑suppressor loci, including Brca1 and E‑cadherin, whose promoters are bound by CtBP1‑containing complexes so that elevated CtBP1 activity correlates with transcriptional downregulation of these genes and reduced epithelial integrity. In breast and other carcinomas, nuclear CtBP1 is frequently detected at elevated levels and is associated with reduced Brca1 and E‑cadherin expression, increased cell migration, and more aggressive disease phenotypes.

Usage Information

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF	FCM
1:1000-1:10000	1:100-1:250	1:100-1:250	1:2000

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

47 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

References

https://pubmed.ncbi.nlm.nih.gov/21681822/
https://pubmed.ncbi.nlm.nih.gov/20818429/

Application Data

WB

Validated by Selleck

Lane 1: SH-SY5Y, Lane 2: Raji, Lane 3: A549, Lane 4: Hela