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Technical Data
| Formula | C26H21N3O3 |
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| Molecular Weight | 423.46 | CAS No. | 55779-48-1 | |
| Solubility (25°C)* | In vitro | DMSO | 84 mg/mL (198.36 mM) | |
| Ethanol | 1 mg/mL (2.36 mM) | |||
| Water | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Preparing Stock Solutions
Biological Activity
| Description | Coelenterazine is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate coelenterazine have been used as bioluminescence donors in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe, and it can also be used in the chemiluminescent detection of peroxynitrite. | |||||
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| In vitro | When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine (8 礸/ml) shows a bioluminescence signal. [1] In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations. [2] |
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| In vivo | In 4T1-implanted mouse, Coelenterazine detects cancer-associated superoxide anion and used to evaluate the role of superoxide anion in cancer cell biology and its role in pathology. [2] |
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| Density | 1.32 g/mL | |||||
Protocol (from reference)
References
Selleck's Coelenterazine has been cited by 6 publications
| RNAi-mediated rheostat for dynamic control of AAV-delivered transgenes [ Nat Commun, 2023, 14(1):1970] | PubMed: 37031257 |
| The molecular evolution of C4-associated gene regulation in the genus Flaveria [ The UWA Research Repository, 2023, ] | PubMed: none |
| Exendin-4 gene modification and microscaffold encapsulation promote self-persistence and antidiabetic activity of MSCs [ Sci Adv, 2021, 7(27)eabi4379] | PubMed: 34215590 |
| Development of a Bioassay for Continuous Monitoring of TWIST1 Activity [ Preprints, 2021, 10.20944/preprints202103.0265.v1] | PubMed: N/A |
| A novel bioassay for quantification of surface Cannabinoid receptor 1 expression [ Sci Rep, 2020, 10(1):18191] | PubMed: 33097803 |
| Tumor treating fields increases membrane permeability in glioblastoma cells [ Cell Death Discov, 2018, 4:113] | PubMed: 30534421 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.