Inhibitor Expert (Inhibitors, Compound Libraries)



Technical Data

Molecular Weight 454.75 Storage powder


in solvent
CAS No. 285986-88-1 Synonyms N/A
Chemical Name N-(1-(4-chlorophenylamino)-1-oxopropan-2-yloxy)-3,5-bis(trifluoromethyl)benzamide
Solubility (25°C) * In vitro DMSO 90 mg/mL (197.91 mM)
Ethanol 4 mg/mL warmed (8.79 mM)
Water Insoluble
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Biological Activity

Description CCG-1423 is a specific RhoA pathway inhibitor, which inhibits SRF-mediated transcription.
RhoA [1]
In vitro CCG-1423 selectively inhibits SRF-mediated transcription activated by Rho pathway signaling, and specifically inhibits LPA-stimulated DNA synthesis. CCG-1423 also selectively inhibits the proliferation of RhoC-overexpressing melanoma cells (A375M2 and SK-Mel-147), and strongly suppresses the Rho-dependent invasion by PC-3 cells. [1] CCG-1423 in combination with LY294002 enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells. [2] In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization, and completely blocks STARS proximal reporter activity. [3] CCG-1423, as a Rho/MRTF/SRF pathway inhibitor, also represses both matrix-stiffness and TGF-beta-induced fibrogenesis in human colonic myofibroblasts. [4]
In vivo

Protocol (Only for Reference)

Cell Assay: [1]
Cell lines Melanoma lines that overexpress RhoC (A375M2 and SK-Mel-147), low RhoC-expressing lines (A375 and SK-Mel-28), transformed (SW962, PC-3, and SKOV-3) and nontransformed (WI-38) cell lines.
Concentrations 300 nM
Incubation Time 72 hours
Method Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.


Customer Product Validation

Data from [Data independently produced by , , Cell Signal, 2017, 31:87-95]

Cells were treated with CCG-1423 for 48 h. CCG-1423 blocked the nuclear translocation of MRTF-A.

Data from [Data independently produced by , , Cellular Signalling, 2017, 31:87-95.]

CCG-1423 blocked the nuclear translocation of MRTF-A.

CCG-1423 has been referenced in publications.



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Chemical Structure

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