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Description
| Specificity | Brd4 Rabbit Recombinant mAb detects endogenous levels of total Brd4. |
|---|---|
| Background | Bromodomain-containing protein 4 (BRD4) is a member of the bromodomain and extraterminal (BET) family proteins characterized by two N-terminal bromodomains and an extraterminal (ET) domain. BRD4 binds to acetylated histones and transcription factors through bromodomains and recruits transcriptional regulators such as positive transcription elongation factor b (P-TEFb) and Mediator complex. BRD4 is involved in the activation of genes involved in cell growth and cell cycle progression. BRD4 also plays a role in different biological processes, including memory formation, mitochondrial oxidative phosphorylation, and DNA damage response. BRD4 suppresses the expression of a subset of autophagy and lysosome genes by binding to the promoter regions under normal growth conditions and that this repression is alleviated in response to certain autophagic stimuli. Inhibition of BRD4 enhances autophagic flux and lysosomal function, which consequently promotes the degradation of pathogenic protein aggregates and confers the resistance to starvation-induced cell death. |
Usage Information
| Application | WB, IF,ELISA | ||||
|---|---|---|---|---|---|
| Dilution |
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| Reactivity | Human Mouse Rat | ||||
| MW (kDa) | 152kDa | ||||
| Source | Rabbit | ||||
| Concentration | 1mg/ml | ||||
| Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||||
| Storage (From the date of receipt) |
Store at –20°C. |
Application Data
WB
Validated by Selleck
IF