|Chemical Name||4-(5-bromopyridin-2-ylamino)-4-oxobutanoic acid|
|Solubility (25°C) *||In vitro||DMSO||54 mg/mL (197.74 mM)|
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Description||Bikinin is an ATP-competitive Arabidopsis GSK-3 inhibitor, and acts as a strong activator of brassinosteroid (BR) signaling.|
|In vitro||Bikinin directly targets BIN2 of Arabidopsis GSK-3s and activates brassinosteroid-dependent gene expression.  Bikinin also inhibits RD26 expression, and inhibits the induction of GUS activity by 150 mM NaCl in the RD26pro:GUS line. |
|In vivo||Bikinin strongly inhibits abscisic acid signaling outputs, and inhibits T180 phosphorylation of SnRK2.3 and its kinase activity in vivo. |
|Kinase Assays||The BIN2 kinase assay with GST-BIN2 and maltose binding protein (MaBP) fused to BES1 (MaBP-BES1) as a substrate is done as reported elsewhere. Kinase assays of the ASKs, AtMPK4 and AtMPK6 with myelin basic protein (MBP), AURORA1 with histone H3, and of the human kinases. Comparable activities of the different ASKs are used. For one particular kinase equal amounts (ranging from 20 to 100 ng depending on the innate kinase activity) are used in the assays with and without bikinin.|
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