BI-9564

Catalog No.S8113 Batch:S811303

Print

Technical Data

Formula

C20H23N3O3

Molecular Weight 353.41 CAS No. 1883429-22-8
Solubility (25°C)* In vitro Ethanol 7 mg/mL (19.8 mM)
DMSO 6 mg/mL (16.97 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description BI-9564 is a selective inhibitor of BRD9 and BRD7 bromodomains with the IC50 of 75 nM and 3.4 µM, respectively.
Targets
BRD9 [1]
(Cell-free assay)
BRD7 [1]
(Cell-free assay)
CECR2 [1]
(Cell-free assay)
5.9 nM(Kd) 73 nM(Kd) 77 nM(Kd)
In vitro Treatment of the panel with BI-9564 results in selective growth inhibition of a significant proportion of AML cell lines tested[1].

Protocol (from reference)

Cell Assay:[1]
  • Cell lines

    MV-4-11 cells

  • Concentrations

    various concentrations(10 nM-100 μM)

  • Incubation Time

    2 h

  • Method

    To 750,000 MV-4-11 cells in 250 µl growth medium (in IMDM, 10 % FBS, GlutaMAX, 25 mM HEPES and 0,1% 2-Mercaptoethanol) per well compound is added at the desired concentration from a 10 mM stock solution using the HP D300 Digital Dispenser. After 2 hours of incubation with the compound, cells are collected by centrifugation, washed in ice cold PBS and lysed in 15 µl of cell extraction buffer. After 30 minutes on ice, nucleic acids are disrupted by sonication. cMYC levels are measured using the human c-Myc (Total) ELISA Kit.

Animal Study:[1]
  • Animal Models

    Female CIEA-NOG mice

  • Dosages

    10 mL/kg

  • Administration

    Oral administration

Selleck's BI-9564 has been cited by 1 publication

GSK2801, a BAZ2/BRD9 Bromodomain Inhibitor, Synergizes with BET Inhibitors to Induce Apoptosis in Triple-Negative Breast Cancer [ Mol Cancer Res, 2019, 17(7):1503-1518] PubMed: 31000582

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.