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Technical Data
| Formula | C14H11ClN6O3 |
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| Molecular Weight | 346.73 | CAS No. | 1644443-47-9 | |
| Solubility (25°C)* | In vitro | DMSO | 5 mg/mL (14.42 mM) | |
| Water | Insoluble | |||
| Ethanol | Insoluble | |||
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
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Biological Activity
| Description | Autophinib is a potent autophagy inhibitor with a novel chemotype with IC50 values of 90 and 40 nM for autophagy in starvation induced autophagy assay and rapamycin induced autophagy assay. The IC50 value for Vps34 is 19 nM in vitro. | ||||||
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| Targets |
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| In vitro | Autophinib inhibits the accumulation of autophagosomes under fed as well as starved conditions, but does not affect the number of autolysosomes. Thus it is not a late stage autophagy inhibitor but an inhibitor of autophagosome formation. Autophinib targets VPS34 but not other lipid kinases, mTOR and TBK1. Autophinib appears to be ATP-competitive, targets VPS34 in cell lysate and induces cellular phenotypes consistent with VPS34 inhibition[1]. |
Protocol (from reference)
| Cell Assay: |
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Selleck's Autophinib has been cited by 8 publications
| Activating autophagy promotes skin regeneration induced by mechanical stretch during tissue expansion [ Burns Trauma, 2024, 12:tkad057] | PubMed: 38328438 |
| Kaempferol promotes non-small cell lung cancer cell autophagy via restricting Met pathway [ Phytomedicine, 2023, 10.1016/j.phymed.2023.155090] | PubMed: 37738907 |
| Genome-wide CRISPR screening identifies TMEM106B as a proviral host factor for SARS-CoV-2 [ Nat Genet, 2021, 53(4):435-444] | PubMed: 33686287 |
| 17β-Estradiol Enhances Porcine Meiosis Resumption from Autophagy-Induced Gap Junction Intercellular Communications and Connexin 43 Phosphorylation via the MEK/ERK Signaling Pathway [ J Agric Food Chem, 2021, 69(40):11847-11855] | PubMed: 34609142 |
| 17β-estradiol improves the developmental ability, inhibits reactive oxygen species levels and apoptosis of porcine oocytes by regulating autophagy events [ J Steroid Biochem Mol Biol, 2021, 209:105826] | PubMed: 33581253 |
| Identification of Required Host Factors for SARS-CoV-2 Infection in Human Cells [ Cell, 2020, S0092-8674(20)31394-5] | PubMed: 33147445 |
| Antileukemic activity of the VPS34-IN1 inhibitor in acute myeloid leukemia [ Oncogenesis, 2020, 9(10):94] | PubMed: 33093450 |
| Stimulation of Hair Growth by Small Molecules that Activate Autophagy [ Cell Rep, 2019, 27(12):3413-3421] | PubMed: 31216464 |
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.