Aurora B Rabbit Recombinant mAb Datasheet

Description

Specificity	Aurora B Rabbit Recombinant mAb detects endogenous levels of total Aurora B.
Background	Aurora B, a member of the Aurora family of serine/threonine protein kinases, is a key player in chromosome segregation. As part of a macromolecular complex known as the chromosome passenger complex, Aurora B concentrates early during mitosis in the proximity of centromeres and kinetochores, the sites of attachment of chromosomes to spindle microtubules. There, it contributes to a number of processes that impart fidelity to cell division, including kinetochore stabilization, kinetochore-microtubule attachment, and the regulation of a surveillance mechanism named the spindle assembly checkpoint. In the regulation of these processes, Aurora B is the fulcrum of a remarkably complex network of interactions that feed back on its localization and activation state. Aurora B, part of the chromosome passenger complex (CPC), is located on the chromosome arms during prophase and at the centromeres during prometaphase and metaphase. Aurora B subsequently localizes to the midbody duringcytokinesis. Aurora B Interacts with p53 During both Interphase and Mitosis, phosphorylates p53 and enhances p53 ubiquitination. Thus, Aurora B negatively regulates p53.

Specificity

Aurora B Rabbit Recombinant mAb detects endogenous levels of total Aurora B.

Background

Aurora B, a member of the Aurora family of serine/threonine protein kinases, is a key player in chromosome segregation. As part of a macromolecular complex known as the chromosome passenger complex, Aurora B concentrates early during mitosis in the proximity of centromeres and kinetochores, the sites of attachment of chromosomes to spindle microtubules. There, it contributes to a number of processes that impart fidelity to cell division, including kinetochore stabilization, kinetochore-microtubule attachment, and the regulation of a surveillance mechanism named the spindle assembly checkpoint. In the regulation of these processes, Aurora B is the fulcrum of a remarkably complex network of interactions that feed back on its localization and activation state. Aurora B, part of the chromosome passenger complex (CPC), is located on the chromosome arms during prophase and at the centromeres during prometaphase and metaphase. Aurora B subsequently localizes to the midbody duringcytokinesis. Aurora B Interacts with p53 During both Interphase and Mitosis, phosphorylates p53 and enhances p53 ubiquitination. Thus, Aurora B negatively regulates p53.

Usage Information

Application

WB, IF,ELISA

Dilution

WB	IF
1:1000	1:50

Reactivity

Human

MW (kDa)

39kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

Application Data

WB

Validated by Selleck

IF