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Specificity | Aurora A Rabbit Recombinant mAb detects endogenous levels of total Aurora A. |
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Background | Aurora kinases, which have been implicated in several vital events in mitosis, represent a protein kinase family highly conserved during evolution. The activity of Aurora kinases is delicately regulated, mainly by phosphorylation and degradation. Deregulation of Aurora kinase activity can result in mitotic abnormality and genetic instability, leading to defects in centrosome function, spindle assembly, chromosome alignment, and cytokinesis. They are crucial for cell cycle control. In Caenorhabditis elegans, Drosophila, and Xenopus, there are two types of Aurora kinases: Aurora-A and Aurora-B. Mammals own at least three Aurora kinases: Aurora-A, Aurora-B, and Aurora-C. Aurora-A localizes to the pericentriolar material from the end of S phase to the beginning of the next G1 and spreads to the pole proximal ends of spindle microtubules during mitosis. In contrast, Aurora-B remains in the nucleus and moves to centromeres from prometaphase to metaphase. Aurora-A is mainly involved in centrosome function, mitotic entry, and spindle assembly, whereas Aurora-B participates in chromatin modification, microtubule-kinetochore attachment, spindle checkpoint, and cytokinesis. |
Application | WB, IHC, IF,ELISA | ||||||
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Dilution |
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Reactivity | Human Mouse | ||||||
MW (kDa) | 46kDa | ||||||
Source | Rabbit | ||||||
Concentration | 1mg/ml | ||||||
Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||||||
Storage (From the date of receipt) |
Store at –20°C. |
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WB
IHC
IF