Datasheet

Biological Description

Specificity	ATP5O Mouse mAb recognizes endogenous levels of total ATP5O protein.
Background	ATP5O, encodes the oligomycin sensitivity-conferring protein (OSCP), is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V) and plays a critical structural and functional role in oxidative phosphorylation. It is located at the top of the peripheral stalk of the ATP synthase complex, linking the membrane-embedded Fo proton channel to the F1 catalytic core in the mitochondrial matrix. By stabilizing this connection, ATP5O ensures the structural integrity of the holoenzyme and facilitates the coupling of proton translocation through Fo with ATP synthesis in F1 during the rotary catalytic cycle. ATP5O is ubiquitously expressed, with highest levels in energy-demanding tissues such as heart, brain, and skeletal muscle. Its proper function is essential for efficient ATP production, and mutations or reduced expression can impair mitochondrial energy metabolism, contributing to conditions like mitochondrial complex V deficiency and age-related decline in bioenergetic capacity.

Specificity

ATP5O Mouse mAb recognizes endogenous levels of total ATP5O protein.

Background

ATP5O, encodes the oligomycin sensitivity-conferring protein (OSCP), is a nuclear-encoded subunit of mitochondrial ATP synthase (Complex V) and plays a critical structural and functional role in oxidative phosphorylation. It is located at the top of the peripheral stalk of the ATP synthase complex, linking the membrane-embedded Fo proton channel to the F1 catalytic core in the mitochondrial matrix. By stabilizing this connection, ATP5O ensures the structural integrity of the holoenzyme and facilitates the coupling of proton translocation through Fo with ATP synthesis in F1 during the rotary catalytic cycle. ATP5O is ubiquitously expressed, with highest levels in energy-demanding tissues such as heart, brain, and skeletal muscle. Its proper function is essential for efficient ATP production, and mutations or reduced expression can impair mitochondrial energy metabolism, contributing to conditions like mitochondrial complex V deficiency and age-related decline in bioenergetic capacity.

Usage Information

Application

WB, IHC, IF, FCM

Dilution

WB	IHC	IF	FCM
0.5 µg/mL	1:500	0.5-1 µg/mL	1 µg/mL

Reactivity

Human, African green monkey, Cow

Source

Mouse

MW

23 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

ATP5O Mouse mAb

Biological Description

Usage Information

References

Application Data