ATP5A1 Rabbit Recombinant mAb

Catalog No.A5288

Description

Specificity	ATP5A1 Rabbit Recombinant mAb detects endogenous level of total ATP5A1.
Background	ATP synthase is a ubiquitous, highly conserved enzyme that catalyses the formation of ATP from ADP and Pi using a unique rotary motor mechanism. The enzyme is located in the inner membrane of mitochondria, in the thylakoid membrane of chloroplasts, and in the plasma membrane of bacteria. ATP synthase is a large multisubunit protein, consisting of an intrinsic membrane domain, Fo, linked through central and side stalks to a globular catalytic domain, F1. The F1 portion consists of three α- and three β-subunits and a single γδɛ-subunit, whereas Fo comprises one a-subunit, two b-subunits and 10-12 c-subunits. ATP5A1 encodes the 553 amino acid α-subunit.

Specificity

ATP5A1 Rabbit Recombinant mAb detects endogenous level of total ATP5A1.

Background

ATP synthase is a ubiquitous, highly conserved enzyme that catalyses the formation of ATP from ADP and Pi using a unique rotary motor mechanism. The enzyme is located in the inner membrane of mitochondria, in the thylakoid membrane of chloroplasts, and in the plasma membrane of bacteria. ATP synthase is a large multisubunit protein, consisting of an intrinsic membrane domain, Fo, linked through central and side stalks to a globular catalytic domain, F1. The F1 portion consists of three α- and three β-subunits and a single γδɛ-subunit, whereas Fo comprises one a-subunit, two b-subunits and 10-12 c-subunits. ATP5A1 encodes the 553 amino acid α-subunit.

Usage Information

Application

WB, IHC,ELISA

Dilution

WB	IHC
1:1000	1:50

Reactivity

Human Mouse Rat

MW (kDa)

55kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

[1] Yoshida M, et al. Nat Rev Mol Cell Biol. 2001, 2(9):669-77.

Application Data

Validated by Selleck

IHC