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Description
| Specificity | Atg4B Rabbit Recombinant mAb detects endogenous level of Atg4B. |
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| Background | Autophagy is an evolutionarily conserved catabolic pathway within all eukaryotic cells that plays a critical housekeeping function to remove senescent or damaged proteins and organelles through lysosome-mediated degradation. Autophagy also plays roles in generating substrates for maintaining cellular energy during times of nutrient insufficiency and hypoxia and in host defenses against intracellular pathogens. Atg4 plays dual roles in the progression of autophagy through the irreversible (proteolytic cleavage) and reversible (lipid deconjugation) modification of Atg8. ATG4B (autophagin-1) is an enzyme that is essential for autophagy in yeast and mammals. ATG4B (autophagin-1) has broad specificity for almost all of the mammalian Atg8 homologues and is the sole enzyme reported to efficiently cleave LC3 precursors and to deconjugate lipid from LC3-PE. Of the four autophagins identified (ATG4A, ATG4B, ATG4C, and ATG4D), ATG4B (autophagin-1) shows the highest catalytic efficiency for cleaving the C terminus of LC3B. |
Usage Information
| Application | WB,ELISA | ||
|---|---|---|---|
| Dilution |
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| Reactivity | Human Mouse Rat | ||
| MW (kDa) | 44kDa | ||
| Source | Rabbit | ||
| Concentration | 1mg/ml | ||
| Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| Storage (From the date of receipt) |
Store at –20°C. |
Application Data
WB