Atg4A Rabbit Recombinant mAb Datasheet

Description

Specificity	Atg4A Rabbit Recombinant mAb detects endogenous level of total Atg4A.
Background	Autophagy is an evolutionarily conserved catabolic pathway within all eukaryotic cells that plays a critical housekeeping function to remove senescent or damaged proteins and organelles through lysosome-mediated degradation. Autophagy also plays roles in generating substrates for maintaining cellular energy during times of nutrient insufficiency and hypoxia and in host defenses against intracellular pathogens. Atg4 plays dual roles in the progression of autophagy through the irreversible (proteolytic cleavage) and reversible (lipid deconjugation) modification of Atg8.

Specificity

Atg4A Rabbit Recombinant mAb detects endogenous level of total Atg4A.

Background

Autophagy is an evolutionarily conserved catabolic pathway within all eukaryotic cells that plays a critical housekeeping function to remove senescent or damaged proteins and organelles through lysosome-mediated degradation. Autophagy also plays roles in generating substrates for maintaining cellular energy during times of nutrient insufficiency and hypoxia and in host defenses against intracellular pathogens. Atg4 plays dual roles in the progression of autophagy through the irreversible (proteolytic cleavage) and reversible (lipid deconjugation) modification of Atg8.

Usage Information

Application

WB, IHC, IF,ELISA

Dilution

WB	IHC	IF
1:1000	1:50	1:50

Reactivity

Human Mouse Rat

MW (kDa)

45kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

[1] Yang Z, et al. J Biol Chem. 2015, 290(44):26549-61.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck

IF