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Description
| Specificity | ASK1 Rabbit Recombinant mAb detects endogenous level of total ASK1. |
|---|---|
| Background | Apoptosis signal-regulating kinase 1 (ASK1) is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family that activates downstream MAP kinases (MAPKs), c-Jun N-terminal kinases (JNKs) and p38 MAPKs, in response to various stresses, such as reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, lipopolysaccharide, and calcium overload. ASK1 also mediates signaling involved in cell fate determination, such as differentiation and survival. It is highly conserved among eukaryotes. The activation of ASK1 is tightly regulated by phosphorylation of a threonine residue (Thr838 in human ASK1) within the activation loop of the kinase domain. The coiled-coil domain in the C-terminus of ASK1 is also essential for the homo-oligomerization and activation of ASK1. |
Usage Information
| Application | WB,ELISA | ||
|---|---|---|---|
| Dilution |
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| Reactivity | Human Mouse | ||
| MW (kDa) | 155kDa | ||
| Source | Rabbit | ||
| Concentration | 1mg/ml | ||
| Storage buffer | 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. | ||
| Storage (From the date of receipt) |
Store at –20°C. |
Application Data
WB