Datasheet

Biological Description

Specificity	Anti-Serotonin transporter Rabbit Antibody [B14A13] detects endogenous levels of total Serotonin transporter protein.
Background	The serotonin transporter (SERT) plays a critical role in regulating serotonergic neurotransmission by clearing serotonin (5-HT) from the synaptic cleft back into presynaptic neurons, effectively terminating serotonin signaling. SERT is a membrane protein with 12 transmembrane helices that couples serotonin reuptake to sodium and chloride ion gradients, operating through a well-defined transport cycle where serotonin first binds to an outward-facing site along with sodium and chloride ions, followed by conformational changes that release serotonin into the cytoplasm. SERT regulates extracellular serotonin concentration, thereby exerting a significant influence over mood, cognition, sleep, and appetite. Regulation of SERT involves multiple pathways: phosphorylation by kinases such as protein kinase C (PKC) and protein kinase G (PKG) modulates its activity and trafficking between the plasma membrane and intracellular compartments; lipid membrane components, including cholesterol and PIP2, affect SERT conformation, oligomerization, and surface expression; and protein-protein interactions fine-tune its localization and function. SERT possesses both a central substrate binding site and an allosteric site that modulates serotonin and inhibitor binding, which is fundamental to the mechanisms of antidepressant drugs like SSRIs (e.g., citalopram, paroxetine), that block reuptake by binding to SERT and increasing serotonin availability. Dysregulation or genetic variants of SERT are associated with neuropsychiatric disorders, including depression, anxiety, and obsessive-compulsive disorder.

Specificity

Anti-Serotonin transporter Rabbit Antibody [B14A13] detects endogenous levels of total Serotonin transporter protein.

Background

The serotonin transporter (SERT) plays a critical role in regulating serotonergic neurotransmission by clearing serotonin (5-HT) from the synaptic cleft back into presynaptic neurons, effectively terminating serotonin signaling. SERT is a membrane protein with 12 transmembrane helices that couples serotonin reuptake to sodium and chloride ion gradients, operating through a well-defined transport cycle where serotonin first binds to an outward-facing site along with sodium and chloride ions, followed by conformational changes that release serotonin into the cytoplasm. SERT regulates extracellular serotonin concentration, thereby exerting a significant influence over mood, cognition, sleep, and appetite. Regulation of SERT involves multiple pathways: phosphorylation by kinases such as protein kinase C (PKC) and protein kinase G (PKG) modulates its activity and trafficking between the plasma membrane and intracellular compartments; lipid membrane components, including cholesterol and PIP2, affect SERT conformation, oligomerization, and surface expression; and protein-protein interactions fine-tune its localization and function. SERT possesses both a central substrate binding site and an allosteric site that modulates serotonin and inhibitor binding, which is fundamental to the mechanisms of antidepressant drugs like SSRIs (e.g., citalopram, paroxetine), that block reuptake by binding to SERT and increasing serotonin availability. Dysregulation or genetic variants of SERT are associated with neuropsychiatric disorders, including depression, anxiety, and obsessive-compulsive disorder.

Usage Information

Application

WB, IHC

Dilution

WB	IHC
1:500 - 1:5000	1:50 - 1:200

Reactivity

Human

Source

Rabbit

MW

71,75 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/27049939/
https://pubmed.ncbi.nlm.nih.gov/30809118/

Anti-Serotonin transporter Rabbit Antibody [B14A13]

Biological Description

Usage Information

References

Application Data