Datasheet

Biological Description

Specificity	Anti-Phospho-PKR (Thr451) Rabbit Antibody [C9J9] detects endogenous levels of PKR protein only when phosphorylated at Thr451.
Background	Protein kinase R (PKR) is an interferon-induced serine/threonine kinase that plays a central role in innate antiviral defense and regulation of cell growth. Structurally, PKR contains two tandem dsRNA-binding motifs at its N-terminus, a flexible linker, and a C-terminal kinase domain that adopts a bilobal fold. PKR expression is upregulated by interferons and it becomes activated upon binding viral dsRNA, which promotes dimerization and autophosphorylation. Among several phosphorylation sites, threonine 451 (Thr451) in the activation loop is critical for enzymatic activity, as mutation of this residue impairs PKR function. Phosphorylation at Thr451, together with Thr446, stabilizes the active conformation, enhances dimerization, and enables PKR to phosphorylate eIF2α at serine 51, thereby blocking translation initiation and suppressing viral replication. Thus, PKR phosphorylation, particularly at Thr451, is essential for its role in antiviral immunity and tumor suppressor functions.

Specificity

Anti-Phospho-PKR (Thr451) Rabbit Antibody [C9J9] detects endogenous levels of PKR protein only when phosphorylated at Thr451.

Background

Protein kinase R (PKR) is an interferon-induced serine/threonine kinase that plays a central role in innate antiviral defense and regulation of cell growth. Structurally, PKR contains two tandem dsRNA-binding motifs at its N-terminus, a flexible linker, and a C-terminal kinase domain that adopts a bilobal fold. PKR expression is upregulated by interferons and it becomes activated upon binding viral dsRNA, which promotes dimerization and autophosphorylation. Among several phosphorylation sites, threonine 451 (Thr451) in the activation loop is critical for enzymatic activity, as mutation of this residue impairs PKR function. Phosphorylation at Thr451, together with Thr446, stabilizes the active conformation, enhances dimerization, and enables PKR to phosphorylate eIF2α at serine 51, thereby blocking translation initiation and suppressing viral replication. Thus, PKR phosphorylation, particularly at Thr451, is essential for its role in antiviral immunity and tumor suppressor functions.

Usage Information

Application

WB

Dilution

WB

1:2000

Reactivity

Human, Pig

Source

Rabbit

MW

62 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/20088595/

Anti-Phospho-PKR (Thr451) Rabbit Antibody [C9J9]

Biological Description

Usage Information

References

Application Data