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Biological Description
| Specificity | Anti-Histone H4 Rabbit Antibody [L22H17] recognizes total endogenous levels of Histone H4 protein. |
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| Background | Histones are highly conserved DNA-binding proteins that form the structural backbone of chromatin in all eukaryotic cells. They are classified into five main groups: H1/H5, H2A, H2B, H3, and H4. The nucleosome, the fundamental repeating unit of chromatin, consists of two copies each of H2A, H2B, H3, and H4, which assemble into a histone octamer. While once thought to provide only a static scaffold for DNA packaging, the nucleosome is now recognized as a dynamic structure subject to diverse post-translational modifications (PTMs) such as acetylation, phosphorylation, methylation, and ubiquitination. Histone acetylation occurs mainly on lysine residues within the amino-terminal tails of histones—H2A (Lys5), H2B (Lys5, 12, 15, 20), H3 (Lys9, 14, 18, 23, 27, 36, 56), and H4 (Lys5, 8, 12, 16). These modifications regulate key processes including histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair. Importantly, acetylation of specific lysines generates docking sites for bromodomain-containing proteins, enabling them to selectively recognize and bind acetylated histones. |
Usage Information
| Application | WB, IHC, IF, ChIP | Dilution |
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| Reactivity | Mouse, Rat, Human, Drosophila melanogaster | ||||||||
| Source | Rabbit | MW | 11 kDa | ||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3 | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||
References
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