Datasheet

Biological Description

Specificity	Anti-Hexokinase II Rabbit Antibody [F23A5] detects endogenous levels of total Hexokinase II protein.
Background	Hexokinase II (HK-II) is a key enzyme catalyzing the phosphorylation of glucose to glucose-6-phosphate, the first and rate-limiting step of glycolysis, predominantly expressed in insulin-sensitive tissues like the heart, skeletal muscle, and adipose tissue. HK-II contains two functional catalytic domains and an N-terminal mitochondrial binding domain, enabling it to associate with the outer mitochondrial membrane, which enhances its enzymatic efficiency and confers anti-apoptotic properties. HK-II integrates metabolic and survival signaling by coupling glucose metabolism with mitochondrial function, thereby enhancing ATP production and protecting against cell death. HK-II expression and activity are tightly regulated by pathways including Akt/mTOR, where Akt-mediated phosphorylation increases mitochondrial targeting, optimizing energy production and cell survival. HK-II binds and inhibits mTORC1 via a conserved TOS motif, triggering protective autophagy independent of its kinase activity under conditions of glucose deprivation. Post-translational modifications such as phosphorylation influence HK-II’s subcellular localization and interaction with signaling networks. Dysregulated HK-II expression is frequently observed in cancers, contributing to the Warburg effect and tumor progression, and is reduced in pathological states like type 1 diabetes.

Specificity

Anti-Hexokinase II Rabbit Antibody [F23A5] detects endogenous levels of total Hexokinase II protein.

Background

Hexokinase II (HK-II) is a key enzyme catalyzing the phosphorylation of glucose to glucose-6-phosphate, the first and rate-limiting step of glycolysis, predominantly expressed in insulin-sensitive tissues like the heart, skeletal muscle, and adipose tissue. HK-II contains two functional catalytic domains and an N-terminal mitochondrial binding domain, enabling it to associate with the outer mitochondrial membrane, which enhances its enzymatic efficiency and confers anti-apoptotic properties. HK-II integrates metabolic and survival signaling by coupling glucose metabolism with mitochondrial function, thereby enhancing ATP production and protecting against cell death. HK-II expression and activity are tightly regulated by pathways including Akt/mTOR, where Akt-mediated phosphorylation increases mitochondrial targeting, optimizing energy production and cell survival. HK-II binds and inhibits mTORC1 via a conserved TOS motif, triggering protective autophagy independent of its kinase activity under conditions of glucose deprivation. Post-translational modifications such as phosphorylation influence HK-II’s subcellular localization and interaction with signaling networks. Dysregulated HK-II expression is frequently observed in cancers, contributing to the Warburg effect and tumor progression, and is reduced in pathological states like type 1 diabetes.

Usage Information

Application

WB, IP, IHC, IF , FCM

Dilution

WB	IP	IHC	IF	FCM
1:1000	1:30	1:500	1:100	1:60

Reactivity

Mouse, Rat, Human

Source

Rabbit

MW

102 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

Exprimental Methods

References

https://pubmed.ncbi.nlm.nih.gov/24462113/
https://pubmed.ncbi.nlm.nih.gov/25323588/

Anti-Hexokinase II Rabbit Antibody [F23A5]

Biological Description

Usage Information

References

Application Data