Angiopoietin 1 Rabbit Recombinant mAb

Catalog No.A5342

Description

Specificity	Angiopoietin 1 Rabbit Recombinant mAb detects endogenous level of total angiopoietin 1.
Background	Angiopoietin-1 (Ang1) is an oligomeric-secreted glycoprotein and a member, with Ang2 and Ang3/4, of the angiopoietin family of growth factors. These ligands bind Tie2, one of two receptor tyrosine kinases, the other being Tie1, that make up the Tie family of receptors expressed primarily in vascular endothelium. The angiopoietin and Tie families have primary roles in the latter stages of vascular development and in adult vasculature, where they control remodeling and stabilization of vessels. Ang1 is required for correct organization and maturation of newly formed vessels and promotes quiescence and structural integrity of adult vasculature. Ang1 and Ang2 bind to Tie2 with similar affinities; however, whereas Ang1 is an agonist, the ability of Ang2 to activate Tie2 appears to depend on the cell type and context. Ang1 is widely expressed and is present in periendothelial cells in quiescent vasculature. Ang1 inhibits endothelial cell apoptosis and stimulates endothelial cell migration.

Specificity

Angiopoietin 1 Rabbit Recombinant mAb detects endogenous level of total angiopoietin 1.

Background

Angiopoietin-1 (Ang1) is an oligomeric-secreted glycoprotein and a member, with Ang2 and Ang3/4, of the angiopoietin family of growth factors. These ligands bind Tie2, one of two receptor tyrosine kinases, the other being Tie1, that make up the Tie family of receptors expressed primarily in vascular endothelium. The angiopoietin and Tie families have primary roles in the latter stages of vascular development and in adult vasculature, where they control remodeling and stabilization of vessels. Ang1 is required for correct organization and maturation of newly formed vessels and promotes quiescence and structural integrity of adult vasculature. Ang1 and Ang2 bind to Tie2 with similar affinities; however, whereas Ang1 is an agonist, the ability of Ang2 to activate Tie2 appears to depend on the cell type and context. Ang1 is widely expressed and is present in periendothelial cells in quiescent vasculature. Ang1 inhibits endothelial cell apoptosis and stimulates endothelial cell migration.

Usage Information

Application

WB, IHC,ELISA

Dilution

WB	IHC
1:1000	1:50

Reactivity

Human Rat

MW (kDa)

57kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

[1] Brindle NP, et al. Circ Res. 2006, 98(8):1014-23.

Application Data

Validated by Selleck

IHC