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Formula | C22H21N3O3S |
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Molecular Weight | 407.49 | CAS No. | 219766-25-3 | |
Solubility (25°C)* | In vitro | DMSO | 37 mg/mL (90.79 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.) |
Description | ANA-12 is a selective TrkB antagonist with Kd of 10 nM and 12 μM for the high and low affinity sites, respectively. | ||
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In vitro | ANA-12 binds directly and selectively to TrkB and inhibits processes downstream of TrkB without altering TrkA and TrkC functions. In nnr5 PC12-TrkB cells, ANA-12 prevents brain-derived neurotrophic factor (BDNF)-induced neurite outgrowth at concentrations as low as 10 nM. [1] In DRG neurons, ANA-12 eliminates the effect of BDNF on increasing inward current. [2] | ||
In vivo | In adult C57BL6/129SveV F1s mice, ANA-12 (0.5 mg/kg, i.p.) decreases TrKB activity in the brain and reduces the anxiety- and depression-related behaviors without affecting the neuron survival. [1] In male C57BL/6 mice, ANA-12 (0.5 mg/kg, i.p.) shows antidepressant-like effects on lipopolysaccharide-induced depression-like behavior. [3] In male Sprague-Dawley rats, ANA-12 (3 μg/dose) blocks the effect of medial nucleus tractus solitarius (mNTS) BDNF on reducing food intake. [4] In male wild-type mice, ANA-12 reverses ethanol intake and induces D3 receptor downregulation, but is ineffective in D3R-/- mice. [5] In male CocSired rats, ANA-12 (0.5 mg/kg, i.p.) reverses the diminished self-administration of cocaine. [6] |
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Data from [Data independently produced by , , Front Cell Neurosci, 2018, doi:10.3389/fncel.2018.00122]
Data from [Data independently produced by , , Am J Physiol Heart Circ Physiol, 2018, doi:10.1152/ajpheart.00245.2018]
Data from [Data independently produced by , , Neuroscience, 2018, 390:151-159]
Hepatic stellate cells maintain liver homeostasis through paracrine neurotrophin-3 signaling that induces hepatocyte proliferation [ Sci Signal, 2023, 16(787):eadf6696] | PubMed: 37253090 |
Periplaneta Americana (L.) extract activates the ERK/CREB/BDNF pathway to promote post-stroke neuroregeneration and recovery of neurological functions in rats [ J Ethnopharmacol, 2023, 10.1016/j.jep.2023.117400] | PubMed: 37952730 |
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD [ Pharmacol Res, 2022, 176:106079] | PubMed: 35026406 |
lncRNA BDNF-AS Attenuates Propofol-Induced Apoptosis in HT22 Cells by Modulating the BDNF/TrkB Pathway [ Mol Neurobiol, 2022, 10.1007/s12035-022-02757-y] | PubMed: 35338452 |
Reduced BDNF expression in the auditory cortex contributed to neonatal pain-induced hearing impairment and dendritic pruning deficiency in mice [ Reg Anesth Pain Med, 2022, rapm-2022-103621] | PubMed: 36384877 |
Atorvastatin inhibits neuronal apoptosis via activating cAMP/PKA/p-CREB/BDNF pathway in hypoxic-ischemic neonatal rats [ FASEB J, 2022, 36(4):e22263] | PubMed: 35303316 |
[ANA- 12 inhibits spinal inflammation and alleviates acute and chronic pain in rats by targeted blocking of BDNF/TrkB signaling] [ Nan Fang Yi Ke Da Xue Xue Bao, 2022, 42(2):232-237] | PubMed: 35365447 |
Melatonin protects inner retinal neurons of newborn mice after hypoxia-ischemia [ J Pineal Res, 2021, 71(1):e12716] | PubMed: 33426650 |
Striatal Shati/Nat8l-BDNF pathways determine the sensitivity to social defeat stress in mice through epigenetic regulation [ Neuropsychopharmacology, 2021, 10.1038/s41386-021-01033-2] | PubMed: 34099867 |
Striatal Shati/Nat8l-BDNF pathways determine the sensitivity to social defeat stress in mice through epigenetic regulation [ Neuropsychopharmacology, 2021, 46(9):1594-1605] | PubMed: 34099867 |
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