Alpha Actinin 4 Rabbit Recombinant mAb

Catalog No.A5471

Description

Specificity	Alpha Actinin 4 Rabbit Recombinant mAb detects endogenous level of total Alpha Actinin 4.
Background	α-Actinins, highly related members of the spectrin superfamily, are ubiquitously expressed. They cross-link actin filaments, with this cross-linking activity regulating the formation of focal adhesions, intracellular tension, and cell migration. Four isoforms have been identified. α-Actinin-2 and -3 are mostly expressed in muscle cells, whereas α-actinin-1 and -4 are present in non-muscle cells. Among these four actinin isoforms, α-actinin-4 (ACTN4) ACTN4 has been shown to play a crucial role in cancer invasion and metastasis and in maintaining normal kidney functions. ACTN4 is phosphorylated upon growth factor stimulation, and this loosens its interaction with actin. ACTN4 is essential for maintaining normal spreading, motility, cellular and nuclear cross-sectional area, and contractility of murine lung fibroblasts by maintaining the balance between transcellular contractility and cell-substratum adhesion.

Specificity

Alpha Actinin 4 Rabbit Recombinant mAb detects endogenous level of total Alpha Actinin 4.

Background

α-Actinins, highly related members of the spectrin superfamily, are ubiquitously expressed. They cross-link actin filaments, with this cross-linking activity regulating the formation of focal adhesions, intracellular tension, and cell migration. Four isoforms have been identified. α-Actinin-2 and -3 are mostly expressed in muscle cells, whereas α-actinin-1 and -4 are present in non-muscle cells. Among these four actinin isoforms, α-actinin-4 (ACTN4) ACTN4 has been shown to play a crucial role in cancer invasion and metastasis and in maintaining normal kidney functions. ACTN4 is phosphorylated upon growth factor stimulation, and this loosens its interaction with actin. ACTN4 is essential for maintaining normal spreading, motility, cellular and nuclear cross-sectional area, and contractility of murine lung fibroblasts by maintaining the balance between transcellular contractility and cell-substratum adhesion.

Usage Information

Application

WB, IHC, IF,ELISA

Dilution

WB	IHC	IF
1:1000	1:50	1:50

Reactivity

Human Mouse Rat

MW (kDa)

105kDa

Source

Rabbit

Concentration

1mg/ml

Storage buffer

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Storage
(From the date of receipt)

Store at –20°C.

References

[1] Shao H, et al. PLoS One. 2010, 5(11):e13921.

Application Data

Validated by Selleck

IHC