ALAS2/ASB C-terminal Rabbit mAb

Catalog No.: F3432

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Biological Description

Specificity ALAS2/ASB C-terminal Rabbit mAb recognizes endogenous levels of total ALAS2/ASB C-terminal protein.
Background ALAS2/ASB C-terminal regions of ALAS2 and ASB family proteins are structurally and functionally distinct, reflecting their specialized roles in different biological processes. ALAS2 (also known as ALAS-E) is an erythroid-specific mitochondrial enzyme essential for heme biosynthesis, with a highly conserved 33-amino acid C-terminal region encoded by exon 11. This C-terminal tail acts as an intrinsic regulatory domain, modulating enzymatic activity and stability—loss-of-function mutations here cause X-linked sideroblastic anemia, while gain-of-function mutations increase catalytic activity, contributing to porphyrias. In contrast, ASB (Ankyrin repeat and SOCS box) proteins, members of the SOCS superfamily, having (∼40 amino acids) is ubiquitously expressed across tissues, possess a C-terminal SOCS box domain that recruits Elongin B/C to form part of an E3 ubiquitin ligase complex, targeting substrates for proteasomal degradation. It regulates diverse cellular processes such as immune signaling and tumorigenesis through targeted substrate ubiquitination. While ALAS2’s C-terminus fine-tunes heme synthesis within erythroid mitochondria, the ASB C-terminus orchestrates ubiquitin-mediated protein turnover, particularly through substrate recognition by N-terminal ankyrin repeats.

Usage Information

Application WB, IP Dilution
WB IP
1:1000 - 1:2000 1:30 - 1:50
Reactivity Human, Mouse, Rat
Source Rabbit MW 65 kDa
Storage Buffer PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃
Storage
(from the date of receipt)		 -20°C (avoid freeze-thaw cycles), 2 years

Application Data