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Biological Description
| Specificity | AKT1 + AKT2 Rabbit mAb recognizes endogenous levels of total AKT1 + AKT2 protein. |
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| Background | AKT1 + AKT2 are closely related serine/threonine kinases within the AKT family, playing crucial roles in diverse cellular processes, including survival, proliferation, and metabolism. Both isoforms share a conserved structural architecture comprising an N-terminal pleckstrin homology (PH) domain for membrane localization via phosphoinositide binding, a central kinase (catalytic) domain responsible for substrate phosphorylation, and a C-terminal regulatory domain that modulates activity. AKT1 is ubiquitously expressed across tissues and is particularly vital for promoting general cell survival, proliferation, and resistance to apoptosis. In contrast, AKT2 is more abundantly expressed in insulin-sensitive tissues such as the liver, skeletal muscle, and adipose tissue, where it plays a predominant role in glucose homeostasis and metabolic regulation. While both isoforms contribute to overlapping signaling pathways, their distinct expression profiles and specific physiological roles highlight isoform-specific functions, with AKT1 being more critical for cellular growth and AKT2 for metabolic control, especially in the context of insulin signaling and glucose uptake. |
Usage Information
| Application | WB, IP, IHC, IF, FCM | Dilution |
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| Reactivity | Human, Mouse, Rat | ||||||||||||
| Source | Rabbit | MW | 56 kDa | ||||||||||
| Storage Buffer | PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN₃ | Storage (from the date of receipt) |
-20°C (avoid freeze-thaw cycles), 2 years | ||||||||||