Ac-DEVD-CHO

Catalog No.S7901 Batch:S790101

Print

Technical Data

Formula

C20H30N4O11
Molecular Weight 502.47 CAS No. 169332-60-9
Solubility (25°C)* In vitro Water 100 mg/mL (199.01 mM)
 
 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Ac-DEVD-CHO (Caspase-3 Inhibitor I, N-Ac-Asp-Glu-Val-Asp-CHO) is a potent aldehyde inhibitor of Group II caspases with Ki values of 0.2 nM and 0.3 nM for for caspase-3 and caspase-7, respectively. Weak inhibition for caspase-2.
Targets
Caspase-3 [1]
(Cell-free assay)		 caspase-8 [1]
(Cell-free assay)		 caspase-7 [1]
(Cell-free assay)		 caspase-10 [1]
(Cell-free assay)		 Caspase-1 [1]
(Cell-free assay)		 View More
230 pM(Ki) 0.92 nM(Ki) 1.6 nM(Ki) 12 nM(Ki) 18 nM(Ki)
In vitro Ac-DEVD-CHO is a potent inhibitor of caspase-3 (Ki = 230 pM). In contrast, caspase-2 cleaves the tetrapeptide substrate poorly and is only weakly inhibited by this compound (Ki = 1.7 μM). Group III caspases are broadly inhibited by this chemical with Ki values ranging from 1 to 300 nM. Inhibition of caspase-3 by this inhibitor in isolated working-heart rat model significantly improves post-ischemic contractile recovery of stunned myocardium, even when given after the onset of ischemia. The mechanism(s) of protection by this compound appear to be independent of apoptosis. Troponin I cleavage was not inhibited by this chemical.
In vivo Ac-DEVD-CHO administered at the time of MI results in a 61% reduction in activated caspase-3 expression in cardiomyocytes (p<0.05), and an 84% reduction in cardiomyocyte apoptosis in the young animals. However, in the aging mice, caspase inhibition had no effect on activated caspase-3 expression or cardiomyocyte apoptosis. This compound suppressed and/or delayed the progression of photoreceptor cell damage in rats and delays disease progression in rd gene-carring mice, which normally develop retinal degeneration early in life.

Protocol (from reference)

Cell Assay:

[3]
  • Cell lines

    MEFs

  • Concentrations

    20, 50 μM

  • Incubation Time

    2 h

  • Method

    Apaf-1−/− cells were treated with the indicated concentration of Ac-DEVD-CHO for 2 h prior to treatment with 4 μM CHX alone or with TNF for 6 h. LEHDase and DEVDase activities were standardized to the lactate dehydrogenase activity in the sample and are presented as percentages of the activity in TNF-plus-CHX-treated cells not treated with this compound (0 μM).
Animal Study:

[4]
  • Animal Models

    C57Bl6 mice

  • Dosages

    3 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/9829999/
  • https://pubmed.ncbi.nlm.nih.gov/11738316/
  • https://pubmed.ncbi.nlm.nih.gov/16966373/
  • https://pubmed.ncbi.nlm.nih.gov/24279384/
  • https://www.jstage.jst.go.jp/article/ahc/36/4/36_4_263/_pdf

Customer Product Validation

Microwave (MW) hyperthermia triggers cell death through caspase-3-mediated apoptosis. H460, PC-9 and H1975 cells were treated with or without Ac-DEVD-CHO for 3 h prior to MW hyperthermia (43°C for 90 min), then allowed to recover at 37°C until 24 h. (A) Cell viability was determined by CCK-8 assay. Data are expressed as the means ± SEM of 3 independent experiments. *P<0.05 vs. the control group, **P<0.01 vs. the control group.

Data from [ , , Int J Oncol, 2018, 53(2):539-550 ]

Selleck's Ac-DEVD-CHO Has Been Cited by 40 Publications

HSPA5 induces autophagy targeting VP2 through the PERK-eIF2α signaling pathway to inhibit SVA replication [ J Virol, 2026, 100(5):e0210325] PubMed: 41973539
Androgen receptors in corticotropin-releasing hormone neurons mediate the sexual dimorphism in restraint-induced thymic atrophy [ Proc Natl Acad Sci U S A, 2025, 122(12):e2426107122] PubMed: 40106355
Cascade-penetrating domino-ferroptosis nano inducer synergizes with sonodynamic therapy for anaplastic thyroid cancer [ Mater Today Bio, 2025, 34:102206] PubMed: 40893363
3A and 2B proteins of SVA play chess game with host restriction factor DDX23 by apoptotic pathway [ J Virol, 2025, 99(10):e0076125] PubMed: 40956085
Activation of human endogenous retroviruses by Sox proteins induces cell apoptosis via the caspase-3 pathway [ Front Microbiol, 2025, 16:1604022] PubMed: 40980324
Resveratrol attenuates trimethyltin chloride-induced cardiac defects via the ROS/Wnt/β-catenin pathway [ Comp Biochem Physiol C Toxicol Pharmacol, 2025, 299:110373] PubMed: 41083115
The resveratrol attenuates reactive oxygen species mediated DNA damage in cardiac malformations caused by 4-tert-octylphenol [ Toxicol Appl Pharmacol, 2025, 498:117284] PubMed: 40023230
AHR/cyp1b1 signaling-mediated extrinsic apoptosis contributes to 6PPDQ-induced cardiac dysfunction in zebrafish embryos [ Environ Pollut, 2024, 345:123467] PubMed: 38311157
Digoxigenin activates autophagy in hepatocellular carcinoma cells by regulating the PI3K/AKT/mTOR pathway [ Cancer Cell Int, 2024, 24(1):405] PubMed: 39696358
The Interaction of HMGB1 with the Proinflammatory TREM-1 Receptor Generates Cytotoxic Lymphocytes Active against HLA-Negative Tumor Cells [ Int J Mol Sci, 2024, 25(1)627] PubMed: 38203798

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.