A-RAF Rabbit Recombinant mAb

Catalog No.A5087

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Description

Specificity A-RAF Rabbit Recombinant mAb detects endogenous levels of total A-RAF.
Background The Ras-Raf-MEK-MAPK (mitogen-activated protein kinase)-signaling pathway plays a key role in the regulation of many cellular functions, including cell proliferation, differentiation and transformation, by transmitting signals from membrane receptors to various cytoplasmic and nuclear targets. One of the key components of this pathway is the serine/threonine protein kinase, Raf. The Raf family kinases consist of three isoforms: A-Raf, B-Raf and C-Raf. B-Raf is highly expressed in neural tissue such as the brain and spinal cord, other tissues however express less B-Raf and its expression in muscle is barely detectable, whereas by contrast C-Raf is ubiquitously expressed with the exception of the brain. A-Raf has more limited tissue distribution, it is most highly expressed in the epididymis and ovaries and it is also present in abundance in liver, uterus and kidney. Notably, some of these are steroid hormone-secreting organs or play an important role in steroid hormone production, so it maybe that A-Raf could be involved in the regulation of steroid hormone synthesis and secretion or that steroid hormones and their receptors may regulate physical functions via A-Raf. A-Raf has a stable or transient localization to the mitochondria and tubular endosomes, which has implicated it in the regulation of apoptosis, energy balance and endocytic membrane traffic. In comparison with B-Raf and C-Raf, A-Raf has the lowest basal kinase activity, the maximal activity of A-RAF is only 20% of that of C-Raf. However, a number of publications have revealed that A-Raf plays an important role in apoptosis, tumorigenesis and resistance to Raf inhibitors. Regarding regulation of A-Raf isoform activity by phosphorylation. A-Raf is weakly activated by oncogenic Ras but more strongly by oncogenic Src and both of these two signals synergize to produce maximal activation. A-Raf was also shown to play a role in regulating the growth of human hematopoietic cells.

Usage Information

Application WB, IF,ELISA
Dilution
WB IF
1:1000 1:50
Reactivity Human
MW (kDa) 68kDa
Source Rabbit
Concentration 1mg/ml
Storage buffer 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Storage
(From the date of receipt)		 Store at –20°C.

Application Data

WB

Validated by Selleck

IF

Validated by Selleck