Crystal Violet

Catalog No.S1917 Batch:S191701

Technical Data

Formula	C₂₅H₃₀ClN₃
Molecular Weight	407.98	CAS No.	548-62-9
Solubility (25°C)*	In vitro	DMSO	82 mg/mL (200.99 mM)
		Water	14 mg/mL (34.31 mM)
		Ethanol	8 mg/mL (19.6 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Crystal violet(Gentian Violet) is a triarylmethane dye.
In vitro	1.Preparation of MitoSOX Red working solution Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution. 2.Sample processing 2.1 Paraffin section: First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH2O 2 min. 2.2 Frozen section: distilled water for 2 min. 2.3 Tissue culture cells: fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min. Note: Other fixatives can be selected according to specific experiments. 3.Crystal violet staining 3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results). 3.2 Wash well in distilled water. 3.3 Dry at room temperature. 3.4 Observe and photograph by light microscope.

Description

Crystal violet(Gentian Violet) is a triarylmethane dye.

In vitro

1.Preparation of MitoSOX Red working solution
Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution.
2.Sample processing
2.1 Paraffin section:
First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH2O 2 min.
2.2 Frozen section:
distilled water for 2 min.
2.3 Tissue culture cells:
fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min.
Note: Other fixatives can be selected according to specific experiments.
3.Crystal violet staining
3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results).
3.2 Wash well in distilled water.
3.3 Dry at room temperature.
3.4 Observe and photograph by light microscope.

Protocol (from reference)

Selleck's Crystal Violet has been cited by 6 publications

Circular RNA circPOFUT1 enhances malignant phenotypes and autophagy-associated chemoresistance via sequestrating miR-488-3p to activate the PLAG1-ATG12 axis in gastric cancer [ Cell Death Dis, 2023, 14(1):10]	PubMed: 36624091
Circ-CCT2 Activates Wnt/β-catenin Signaling to Facilitate Hepatoblastoma Development by Stabilizing PTBP1 mRNA [ Cell Mol Gastroenterol Hepatol, 2023, 10.1016/j.jcmgh.2023.10.004]	PubMed: 37866478
Thalidomide suppresses angiogenesis and immune evasion via lncRNA FGD5-AS1/miR-454-3p/ZEB1 axis-mediated VEGFA expression and PD-1/PD-L1 checkpoint in NSCLC [ Chem Biol Interact, 2021, 349:109652]	PubMed: 34520751
PTEN Inhibition Accelerates Corneal Endothelial Wound Healing through Increased Endothelial Cell Division and Migration [ Invest Ophthalmol Vis Sci, 2020, 61(8):19]	PubMed: 32667999
Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression. [ Neurosci Bull, 2019, 10.1007/s12264-019-00436-y]	PubMed: 31646435
Apoptotic effect of fluoxetine through the endoplasmic reticulum stress pathway in the human gastric cancer cell line AGS. [ Naunyn Schmiedebergs Arch Pharmacol, 2019, 10.1007/s00210-019-01739-7]	PubMed: 31707450

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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