Alizarin

Catalog No.S2526 Batch:S252601

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Technical Data

Formula

C14H8O4
Molecular Weight 240.21 CAS No. 72-48-0
Solubility (25°C)* In vitro DMSO 48 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.2mg/ml (5.00mM) Taking the 1 mL working solution as an example, add 50 μL of 24 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.34mg/ml (1.42mM) Taking the 1 mL working solution as an example, add 50 μL of 6.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Alizarin is a red coloring mordant dye extracted from the roots of madder plants (Rubia tinctorum L). This compound strongly inhibits P450 isoform CYP1A1, CYP1A2, and CYP1B1 with IC50 of 6.2 μM, 10.0 μM, and 2.7 μM, respectively in an enzymatic assay. There are also literature reports this chemical increases the CYP1A1 enzymatic activity and acts as an agonist to the AHR receptor in HepG2 cells.
Targets
AhR [2] CYP1B1 [1] CYP1A1 [1] CYP1A2 [1]
2.7 μM 6.2 μM 10.0 μM
In vitro Alizarin weakly inhibits CYP2A6 and CYP2E1. This compound shows competitive inhibition against CYP1B1 with Ki of 0.5 μM. It reduces the mutagenicity of MeIQx, which induced by each CYP1A2 or CYP1B1, while does not effectively reduce the mutation induced by B[a]P. [1] This chemical exhibits antioxidants against iodophenol-derived phenoxyl radicals, superoxide anion radicals and lipid peroxidation in rat liver microsomes. [2]
In vivo Alizarin also reduces the hepatic content of thiobarbituric acid-reactive substances and the serum level of alanine aminotransferase in poisoned animals. [2]

Protocol (from reference)

References

  • https://pubmed.ncbi.nlm.nih.gov/12379470/
  • http://informahealthcare.com/doi/abs/10.1080/13590849762664

Selleck's Alizarin Has Been Cited by 4 Publications

Nuclear DNA and Mitochondrial Damage of the Cooked Meat Carcinogen 2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in Human Neuroblastoma Cells [ Chem Res Toxicol, 2023, 36(8):1361-1373] PubMed: 37421305
NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma [ BMC Med, 2022, 20(1):365] PubMed: 36258210
Phosphoglycerate Mutase 1 Activates DNA Damage Repair via Regulation of WIP1 Activity. [ Cell Rep, 2020, 14;31(2):107518] PubMed: 32294440
Silicate-based bioceramic scaffolds for dual-lineage regeneration of osteochondral defect. [ Biomaterials, 2019, 192:323-333] PubMed: 30468999

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.