3-chloro-5-hydroxybenzoic Acid

Catalog No.S5400 Batch:S540001

Technical Data

Formula

C₇H₅ClO₃

Molecular Weight

172.57

CAS No.

53984-36-4

Solubility (25°C)*

In vitro

DMSO

34 mg/mL (197.02 mM)

Water

Insoluble

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.7mg/ml (9.85mM)	Taking the 1 mL working solution as an example, add 50 μL of 34 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.29mg/ml (1.68mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	3-chloro-5-hydroxybenzoic Acid is a selective agonist of the lactate receptor GPR81.
In vitro	3-Chloro-5-hydroxybenzoic acid shows potency against human (EC⁵⁰=16 μM), monkey (EC⁵⁰=17 μM), dog (EC⁵⁰=67 μM), rat (EC⁵⁰=7 μM), mouse (EC⁵⁰=22 μM), and hamster GPR81 (EC⁵⁰=27 μM).
In vivo	3-Chloro-5-hydroxybenzoic acid (30 mg/kg, 100 mg/kg, 300 mg/kg) shows significant reductions in free fatty acids, resulting in a minimum efficacious dose of 30 mg/kg.

Protocol (from reference)

References

https://pubmed.ncbi.nlm.nih.gov/24900524/

Selleck's 3-chloro-5-hydroxybenzoic Acid Has Been Cited by 6 Publications

The lactate-GPR81-PKA axis regulates exercise-induced central fatigue in male mice [ American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2026, Online ahead of print]	PubMed: 41500517.0
Macrophages facilitate tumor cell PD-L1 expression via an IL-1β-centered loop to attenuate immune checkpoint blockade [ MedComm (2020), 2023, 4(2):e242]	PubMed: 37009412
HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement [ MedComm, 2023, e242]	PubMed: 41614058.0
Lactate Mediates High-Intensity Interval Training—Induced Promotion of Hippocampal Mitochondrial Function through the GPR81-ERK1/2 Pathway [ Antioxidants, 2023, 2087]	PubMed: 38136207.0
Lactate Mediates High-Intensity Interval Training-Induced Promotion of Hippocampal Mitochondrial Function through the GPR81-ERK1/2 Pathway [ Antioxidants (Basel), 2023, 12(12)2087]	PubMed: 38136207
Activation of brain lactate receptor GPR81 aggravates exercise-induced central fatigue [ American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2022, R746-R756]	PubMed: 36189986.0

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.