Brachyury/Bry Antibody [B3G11] | Primary Antibodies

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:1000 IHC1:8000 IF1:1000 FCM1:400

Application
WB, IP, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit Monoclonal Antibody

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
47 kDa 43 kDa,49 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight. Post-translational modifications(e.g., phosphorylation, glycosylation); Splice variants and isoforms; Relative charge; Multimerization.

Datasheet & SDS

Biological Description

Specificity
Brachyury/Bry Antibody [B3G11] detects endogenous levels of total Brachyury/Bry protein.

Clone
B3G11

Synonym(s)
TBXT, T-box transcription factor T, Brachyury protein, Protein T

Background
Brachyury (Bry, T) is an evolutionarily conserved T-box transcription factor that defines posterior mesoderm and notochord identity and integrates multiple developmental signaling pathways with lineage-specific gene programs in early embryos and notochord-derived tissues. The protein contains an N‑terminal T‑box DNA-binding domain that recognizes a palindromic T-site motif and a C‑terminal transactivation region that recruits transcriptional machinery and cofactors to activate target genes involved in mesoderm specification, morphogenetic movement, and axial elongation. During gastrulation, Brachyury expression at the primitive streak and along the forming axis marks progenitors that contribute to axial and paraxial mesoderm, and genome-wide binding maps define direct regulation of genes controlling cell motility, extracellular matrix organization, and cytoskeletal remodeling, linking this factor to coordinated tissue movements and germ layer patterning. Transcriptional network analysis positions Brachyury as a central activator that connects developmental signaling inputs to downstream regulators of hematopoietic, muscle, and endoderm-related fates, and integrates into feedback loops with Foxa2 and Sox17 that align lineage commitment with streak formation and axial extension. Upstream, Brachyury expression responds to gradients of morphogens such as Wnt and BMP that define posterior identity, while downstream targets encompass growth factors, adhesion molecules, and matrix components that shape cell migration paths, cohesion, and compartment boundaries in the emerging mesoderm. Brachyury maintains extracellular matrix homeostasis by binding a defined motif in the Smad3 promoter, increasing Smad3 transcription and protein abundance, and thereby supporting Smad3 phosphorylation and TGF‑β–Smad3–dependent expression of collagen II and aggrecan, which stabilizes disc matrix structure. This axis places Brachyury as a transcriptional relay between TGF‑β signaling and matrix gene expression in nucleus pulposus, with its reduced expression correlating with impaired matrix synthesis and disc degeneration phenotypes. Structural organization of the T‑box domain supports sequence-specific DNA recognition and dimerization properties shared across the T‑box family, while the variable C‑terminal portion encodes context-dependent regulatory motifs that adjust transactivation strength across embryonic and postnatal tissues. In human disease, persistent or ectopic Brachyury expression characterizes notochordal tumors such as chordoma and also appears in subsets of carcinomas, where its developmental roles in motility and matrix regulation align with epithelial–mesenchymal transition features, invasive behavior, and cancer stem-like traits described in mechanistic tumor-focused literature.

Background

Brachyury (Bry, T) is an evolutionarily conserved T-box transcription factor that defines posterior mesoderm and notochord identity and integrates multiple developmental signaling pathways with lineage-specific gene programs in early embryos and notochord-derived tissues. The protein contains an N‑terminal T‑box DNA-binding domain that recognizes a palindromic T-site motif and a C‑terminal transactivation region that recruits transcriptional machinery and cofactors to activate target genes involved in mesoderm specification, morphogenetic movement, and axial elongation. During gastrulation, Brachyury expression at the primitive streak and along the forming axis marks progenitors that contribute to axial and paraxial mesoderm, and genome-wide binding maps define direct regulation of genes controlling cell motility, extracellular matrix organization, and cytoskeletal remodeling, linking this factor to coordinated tissue movements and germ layer patterning. Transcriptional network analysis positions Brachyury as a central activator that connects developmental signaling inputs to downstream regulators of hematopoietic, muscle, and endoderm-related fates, and integrates into feedback loops with Foxa2 and Sox17 that align lineage commitment with streak formation and axial extension. Upstream, Brachyury expression responds to gradients of morphogens such as Wnt and BMP that define posterior identity, while downstream targets encompass growth factors, adhesion molecules, and matrix components that shape cell migration paths, cohesion, and compartment boundaries in the emerging mesoderm. Brachyury maintains extracellular matrix homeostasis by binding a defined motif in the Smad3 promoter, increasing Smad3 transcription and protein abundance, and thereby supporting Smad3 phosphorylation and TGF‑β–Smad3–dependent expression of collagen II and aggrecan, which stabilizes disc matrix structure. This axis places Brachyury as a transcriptional relay between TGF‑β signaling and matrix gene expression in nucleus pulposus, with its reduced expression correlating with impaired matrix synthesis and disc degeneration phenotypes. Structural organization of the T‑box domain supports sequence-specific DNA recognition and dimerization properties shared across the T‑box family, while the variable C‑terminal portion encodes context-dependent regulatory motifs that adjust transactivation strength across embryonic and postnatal tissues. In human disease, persistent or ectopic Brachyury expression characterizes notochordal tumors such as chordoma and also appears in subsets of carcinomas, where its developmental roles in motility and matrix regulation align with epithelial–mesenchymal transition features, invasive behavior, and cancer stem-like traits described in mechanistic tumor-focused literature.

References
https://pubmed.ncbi.nlm.nih.gov/38690917/ https://pubmed.ncbi.nlm.nih.gov/24616493/

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%