Name: Trolox
Brand: Selleck Chemicals
SKU: S3665
Rating: 5 (5 reviews)

Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.

Trolox Chemical Structure

CAS: 53188-07-1

Selleck's Trolox has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 99.98%

99.98

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Biological Activity

Description	Trolox is a cell-permeable, water-soluble derivative of vitamin E with potent antioxidant properties.

In vitro
In vitro	Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-Carboxylic Acid) is a vitamin E analog and a direct scavenger of peroxyl and alkoxyl radicals. Trolox impairs PT4 cell survival, has minor effects on ROS levels and causes wide deregulation of cell cycle genes^[1]. Trolox enhances As2O3-mediated apoptosis in APL, myeloma, and breast cancer cells. Trolox inhibits DNA damage formation induced by singlet oxygen in human lymphoblast WTK-1 cells and protects red blood cells during photodynamic treatment^[2].
Cell Research	Cell lines	NB4, IM9, and AsR2 cells
	Concentrations	100 μM
	Incubation Time	6 days
	Method	NB4, IM9, and AsR2 cells are seeded at 1 × 10⁵ cells/mL in 24-well plates. Cells are treated with various concentrations of As2O3 or doxorubicin, alone or in combination with 100 μM trolox for 6 days. Viable cells are counted by trypan blue exclusion on days 1, 3, and 6. All cells are maintained at a density lower than 1 × 10⁶ cells/mL through dilution as required, and media ± treatment is replaced every third day. MCF-7, MDA-231, and T47D are seeded in 24-well plates at a density of 4000 cells/well. The next day, fresh media containing As2O3 ± trolox is added. On the days indicated, cells are fixed in 10% trichloroacetic acid and subsequently stained with sulforhodamine B (SRB). Bound SRB is solubilized in 10 mM unbuffered Tris (tris(hydroxymethyl)aminomethane), and optical density is measured at 570 nm in a microplate reader.

In Vivo
In vivo	After cerebral ischemia reperfusion, Trolox prescription increases anti-apoptotic proteins and decreased proapoptotic proteins thus protects neurons of the hippocampus and caused improvement of memory^[3].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05937737	Completed	Type 2 Diabetes	Hacettepe University	April 20 2020	Not Applicable
NCT04716764	Unknown status	Breast Cancer\|Female	Necmettin Erbakan University\|Hacettepe University\|Turkish Society of Medical Oncology	March 3 2020	--
NCT04053569	Active not recruiting	Metabolic Syndrome Protection Against	Azienda Ospedaliera Specializzata in Gastroenterologia Saverio de Bellis	October 1 2019	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	250.29	Formula	C₁₄H₁₈O₄
CAS No.	53188-07-1	SDF	Download Trolox SDF
Smiles	CC1=C(C2=C(CCC(O2)(C)C(=O)O)C(=C1O)C)C
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 50 mg/mL ( (199.76 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 50 mg/mL

Water : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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