SEA0400

SEA0400 is a selective and potent inhibitor of the Na+-Ca2+ exchanger (NCX) that inhibits Na+-dependent Ca2+ uptake in cultured neurons, astrocytes, and microglia with IC50 of 33 nM, 5.0 nM and 8.3 nM, respectively. SEA0400 prevents sodium nitroprusside (SNP) from increasing ERK and p38 MAPK phosphorylation and production of reactive oxygen species (ROS) in an extracellular Ca(2+)-dependent manner.

SEA0400 Chemical Structure

SEA0400 Chemical Structure

CAS: 223104-29-8

Purity & Quality Control

Batch: S692001 DMSO] 74 mg/mL] false] Ethanol] 74 mg/mL] false] Water] Insoluble] false Purity: 99.96%
99.96

SEA0400 Related Products

Choose Selective Calcium Channel Inhibitors

Biological Activity

Description SEA0400 is a selective and potent inhibitor of the Na+-Ca2+ exchanger (NCX) that inhibits Na+-dependent Ca2+ uptake in cultured neurons, astrocytes, and microglia with IC50 of 33 nM, 5.0 nM and 8.3 nM, respectively. SEA0400 prevents sodium nitroprusside (SNP) from increasing ERK and p38 MAPK phosphorylation and production of reactive oxygen species (ROS) in an extracellular Ca(2+)-dependent manner.
Targets
p38 MAPK [2] ERK [2] ROS [2] NCX [1]
(in cultured astrocytes)
NCX [1]
(in cultured microglia)
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5.0 nM 8.3 nM

Chemical Information & Solubility

Molecular Weight 371.38 Formula

C21H19F2NO3

CAS No. 223104-29-8 SDF --
Smiles CCOC1=CC=C(OC2=CC=C(OCC3=C(F)C=CC(=C3)F)C=C2)C(=C1)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 74 mg/mL ( (199.25 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 74 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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