Home Neuronal Signaling Dopamine Receptor antagonist 6-OHDA (Oxidopamine hydrobromide) For research use only.

6-OHDA (Oxidopamine hydrobromide)

Synonyms: 6-hydroxydopamine; 2,4,5-trihydroxyphenethylamine

6-OHDA (Oxidopamine hydrobromide) is a neurotoxic synthetic organic compound that acts as an antagonist of the neurotransmitter dopamine with potential antineoplastic activity. Solutions are unstable and should be fresh-prepared.

6-OHDA (Oxidopamine hydrobromide) Chemical Structure

6-OHDA (Oxidopamine hydrobromide) Chemical Structure

CAS: 636-00-0

Selleck's 6-OHDA (Oxidopamine hydrobromide) has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.99%
99.99

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Signaling Pathway

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Biological Activity

Description 6-OHDA (Oxidopamine hydrobromide) is a neurotoxic synthetic organic compound that acts as an antagonist of the neurotransmitter dopamine with potential antineoplastic activity. Solutions are unstable and should be fresh-prepared.
In vitro
In vitro

In PC12 cells, 6-hydroxydopamine (6-OHDA) results in the activation of caspases and apoptosis, also induces superoxide generation, Bid cleavage and mitochondrial membrane depolarization.In addition, Akt phosphorylation that is favorable to cell survival is decreased and p38 MAPK phosphorylation is increased by 6-OHDA.[1]
Cell Research Cell lines PC12 cells
Concentrations 75 μM
Incubation Time 12 h
Method

PC12 cells (2×105cells/ml) are stained with Hoechst33342 after incubation for 12h with or without 75 μM 6-OHDA and observed under fluorescence microscopy. Cells are treated with various concentrations of 6-OHDA for indicated times and stained with Hoechst33342. Total cells (500-1000 cells) and chromatin condensed cells are counted in the same field, and percentage of chromatin condensation is calculated.
In Vivo
In vivo

Oxidopamine hydrobromide (6-OHDA hydrobromide), an antagonist of the neurotransmitter dopamine, is a widely used neurotoxin that selectively destroys dopaminergic neurons.[2]
Animal Research Animal Models female Sprague-Dawley rats
Dosages 12 mM
Administration i.s.

Chemical Information & Solubility

Molecular Weight 250.09 Formula

C8H11NO3.HBr
CAS No. 636-00-0 SDF --
Smiles C1=C(C(=CC(=C1O)O)O)CCN.Br
Storage (From the date of receipt) 3 years-20°C powder Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.

In vitro
Batch:

DMSO : 50 mg/mL ( (199.92 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 50 mg/mL

Ethanol : 13 mg/mL


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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