L-NAME HCl

Catalog No.S2877

L-NAME HCl Chemical Structure

Molecular Weight(MW): 269.69

L-NAME is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively.

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3 Customer Reviews

  • (B) NO levels in cucumber leaves under normal conditions. L, L-NAME; N, NaN3; C, wild type and H2O was used as a control. The mean values of three independent samples and standard errors are shown, and the same letter above the column indicates no significant differences at P < 0.05.

    Front Plant Sci, 2016, 7:1652.. L-NAME HCl purchased from Selleck.

    (C) HOSS1 cells were grown in soft agar, in the presence or absence of L-NAME (10 mM), and treated as indicated with drugs, as indicated, for 6 h and 12 h. Cells were subjected to electrophoresis and stained. The tail moments of cells were measured and presented below each image (n = 4, ± SEM) *P < 0.05 less than corresponding value in vehicle-treated cells.

    Cancer Biol Ther, 2014, 15(6):758-67. . L-NAME HCl purchased from Selleck.

  • (E) Western blot analysis of p-eNOS expression after 1 mM metformin and/or 1 mM l-NAME treatment (n = 3). (F) NO production measurement via Griess method after 1 mM metformin and/or 1 mM l-NAME treatment (n = 3). (G,H) Q-PCR analysis of vWF and CD31 expression after 1 mM metformin and/or 1 mM l-NAME treatment (n = 3). **p < 0.01 when comparison was performed between groups, ***p < 0.001 between groups.

    Biochem Biophys Res Commun, 2015, 465(4):803-9. . L-NAME HCl purchased from Selleck.

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Choose Selective NOS Inhibitors

Biological Activity

Description L-NAME is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively.
Targets
nNOS [1]
(Cell-free assay)
eNOS [1]
(Cell-free assay)
15 nM(Ki) 39 nM(Ki)
In vitro

NG-nitro-L-arginine methyl ester (L-NAME; at 0.1-100 mM) causes concentration-dependent inhibition of the Ca2(+)-dependent endothelial NO synthase from porcine aortae. L-NAME causes an endothelium-dependent contraction and an inhibition of the endothelium-dependent relaxation induced by acetylcholine (ACh) in aortic rings. [2] In another research, Viability of rMC-1 cells or BREC in 25 mM glucose is significantly less than at 5 mM glucose, and this cell death is inhibited by l-NAME in both cell types. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse BV2 cells Mk\lSpVv[3Srb36gZZN{[Xl? MVuyOEBp MoqwTY5pcWKrdHnvckBw\iCQaYTybYMhd3irZHWgd5lvfGijc3WgZYN1cX[rdImgbY4hdW:3c3WgRnYzKGOnbHzzJIF{e2W|c3XkJIF{KEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDy[YFkfGmxbjygTWM2OD1zOD65JO69VQ>? M3LrbFIyOzd5M{[4
mouse RAW264.7 cells M{nTemZ2dmO2aX;uJIF{e2G7 NVjGRVNvOTdvMkCgbC=> NUi3dHY6SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFnGUk1o[W2vYT;MVHMue3SrbYXsZZRm\CCwaYTybYMhd3irZHWgdJJw\HWldHnvckBi\nSncjCxO{B1dyB{MDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUK3MlE{KM7:TR?= NHLENFQyQTN3OUC2PC=>

... Click to View More Cell Line Experimental Data

In vivo L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2]

Protocol

Kinase Assay:[1]
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Enzyme Assay:

The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated.
Cell Research:[3]
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  • Cell lines: rMC-1 cells
  • Concentrations: 1 mM
  • Incubation Time: 5 days
  • Method: rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days.
    (Only for Reference)
Animal Research:[2]
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  • Animal Models: Male Wistar rats
  • Formulation: N/A
  • Dosages: 100 mg/kg
  • Administration: i.v.
    (Only for Reference)

Solubility (25°C)

In vitro Water 54 mg/mL (200.22 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
Saline
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 269.69
Formula

C7H15N5O4.HCl

CAS No. 51298-62-5
Storage powder
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00835224 Completed Orthostatic Hypotension|Spinal Cord Injury VA Office of Research and Development May 2010 Phase 2
NCT00753948 Completed Tetraplegia|Asthma VA Office of Research and Development December 2006 Phase 2|Phase 3
NCT00603720 Completed Cardiovascular Diseases Washington University School of Medicine|National Institute on Aging (NIA) September 2005 --
NCT00237770 Completed Hypotension|Spinal Cord Injury VA Office of Research and Development June 2003 Phase 2|Phase 3

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID