Home Immunology & Inflammation NOS inhibitor - COX inhibitor - NF-κB inhibitor Hederagenin For research use only.

Hederagenin

Synonyms: Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E

Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. Hederagenin inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.

Hederagenin Chemical Structure

Hederagenin Chemical Structure

CAS: 465-99-6

Purity & Quality Control

Batch: S389901 DMSO] 94 mg/mL] false] ] ] false] ] ] false Purity: 98%
98

Hederagenin Related Products

Choose Selective NOS Inhibitors

Biological Activity

Description Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. Hederagenin inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.
Targets
iNOS [2] COX-2 [2] NF-κB [2]
In vitro
In vitro Hederagenin could induce apoptosis of LoVo cells through the mitochondrial apoptotic pathway. Hederagenin could induce the up-regulation of Bax and down-regulation of Bcl-2, Bcl-xL and Survivin, the release of cytochrome c, followed by procaspase-9, procaspase-3 and proPARP cleavage[1]. Hederagenin exhibits antiinflammatory activities via decreasing production of pro-inflammatory mediators (NO, PGE2, TNF-α, IL-1β, and IL-6) through suppression of the pathways of NF-κB in LPS-induced RAW264.7 cells[2].
Cell Research Cell lines LoVo cells
Concentrations 0.25, 0.5, 1.0, 2.0 and 4.0 μM
Incubation Time 24 or 48 h
Method LoVo cells are seeded into 96-well plates at a density of 10,000 cells/well. After being treated with 0.25, 0.5, 1.0, 2.0 and 4.0 μM hederagenin for 24 or 48 h, the medium is discarded and treated with 20 μl MTT (5 mg/ml). After incubation for 4 h at 37°C, the 100 μl DMSO is added to each well to dissolve the resultant formazan crystals. Absorbance is detected at 490 nm.

Chemical Information & Solubility

Molecular Weight 472.70 Formula

C30H48O4
CAS No. 465-99-6 SDF --
Smiles CC1(CCC2(CCC3(C(=CCC4C3(CCC5C4(CCC(C5(C)CO)O)C)C)C2C1)C)C(=O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 94 mg/mL ( (198.85 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Hederagenin | Hederagenin supplier | purchase Hederagenin | Hederagenin cost | Hederagenin manufacturer | order Hederagenin | Hederagenin distributor